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1. WO2022042706 - METHOD FOR DETERMINING GENERATION OF HOOK EFFECT, AND IMMUNOASSAY METHOD AND DEVICE

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Publication Number WO/2022/042706
Publication Date 03.03.2022
International Application No. PCT/CN2021/115144
International Filing Date 27.08.2021
IPC
G01N 33/53 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/538 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
537with separation of immune complex from unbound antigen or antibody
538by sorbent column, particles or resin strip
G01N 33/557 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
557using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
G01N 21/17 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
CPC
G01N 21/17
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
G01N 21/82
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
82producing a precipitate or turbidity
G01N 33/53
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/538
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
537with separation of immune complex from unbound antigen or antibody
538by sorbent column, particles or resin strip ; , i.e. sorbent materials
G01N 33/557
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
557using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
Applicants
  • 深圳市帝迈生物技术有限公司 SHENZHEN DYMIND BIOTECHNOLOGY CO. , LTD [CN]/[CN]
Inventors
  • 牛建会 NIU, Jianhui
Agents
  • 深圳市威世博知识产权代理事务所（普通合伙） CHINA WISPRO INTELLECTUAL PROPERTY LLP.
Priority Data
202010887720.X28.08.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR DETERMINING GENERATION OF HOOK EFFECT, AND IMMUNOASSAY METHOD AND DEVICE
(FR) PROCÉDÉ POUR DÉTERMINER LA GÉNÉRATION D'UN EFFET CROCHET, ET PROCÉDÉ ET DISPOSITIF D'IMMUNO-ESSAI
(ZH) 判断产生钩状效应的方法、免疫分析方法以及装置
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Abstract
(EN) A method for determining the generation of a hook effect, and an immunoassay method and device. The method comprises: obtaining an optical signal generated by performing immunoturbidimetric detection on a target solution, wherein the target solution is formed by adding an antigen solution to an antibody solution, and an antibody in the antibody solution combines with an antigen in the antigen solution to form an immune complex; converting the optical signal into an electrical signal; and when an initial value of the electrical signal meets a preset requirement, determining that a hook effect is generated in the target solution. By means of the foregoing manner, it is possible to quickly detect and confirm whether a hook effect is generated in the target solution.
(FR) La présente invention concerne un procédé pour déterminer la génération d'un effet crochet, ainsi qu'un procédé et un dispositif d'immuno-essai. Le procédé consiste à : obtenir un signal optique généré en effectuant une détection immunoturbidimétrique sur une solution cible, la solution cible étant formée en ajoutant une solution d'antigène à une solution d'anticorps, et un anticorps dans la solution d'anticorps se combinant avec un antigène dans la solution d'antigène pour former un complexe immun ; convertir le signal optique en un signal électrique ; et, lorsqu'une valeur initiale du signal électrique satisfait une exigence prédéfinie, déterminer qu'un effet crochet est généré dans la solution cible. Au moyen de la manière précédente, il est possible de détecter et de confirmer rapidement si un effet crochet est ou non généré dans la solution cible.
(ZH) 一种判断产生钩状效应的方法、免疫分析方法以及装置，该方法包括：获取对目标溶液进行免疫比浊检测产生的光信号；其中，目标溶液由抗体溶液中加入抗原溶液形成，抗体溶液中的抗体与抗原溶液中的抗原结合形成免疫复合物；将光信号转化为电信号；在电信号的初始值满足预设要求时，确定目标溶液产生钩状效应。通过这样的方式，能够对目标溶液是否产生钩状效应进行快速地检测确认。
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