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1. WO2022026451 - CELL CULTURE MEDIA AND METHODS OF MAKING AND USING THE SAME

Publication Number WO/2022/026451
Publication Date 03.02.2022
International Application No. PCT/US2021/043289
International Filing Date 27.07.2021
IPC
C12N 5/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
B01D 61/02 2006.1
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
61Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
02Reverse osmosis; Hyperfiltration
CPC
B01D 2311/12
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
2311Details relating to membrane separation process operations and control
12Addition of chemical agents
B01D 2321/168
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
2321Details relating to membrane cleaning, regeneration, sterilization or to the prevention of fouling
16Use of chemical agents
168Use of other chemical agents
B01D 61/027
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
61Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis, ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
02Reverse osmosis; Hyperfiltration ; ; Nanofiltration
027Nanofiltration
B01D 65/08
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
65Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
08Prevention of membrane fouling or of concentration polarisation
C12N 2501/105
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
10Growth factors
105Insulin-like growth factors [IGF]
C12N 5/0068
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0068General culture methods using substrates
Applicants
  • AMGEN INC. [US]/[US]
Inventors
  • MARCHAND, Nicholas
  • BOLTON, Glen
  • GALL, Sara Ann
  • WU, Jian
Agents
  • WU, Henry P.
Priority Data
63/058,92130.07.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) CELL CULTURE MEDIA AND METHODS OF MAKING AND USING THE SAME
(FR) MILIEUX DE CULTURE CELLULAIRE ET PROCÉDÉS DE FABRICATION ET D'UTILISATION DE CEUX-CI ET D'UTILISATION
Abstract
(EN) Presented herein are methods for preparing and treating mammalian cell culture media through the addition of low levels of poloxamer and nanofiltration. Low levels of poloxamer improved flux and retained greater levels of a growth factor.
(FR) L'invention concerne des procédés de préparation et de traitement de milieux de culture cellulaire de mammifère par l'ajout de faibles niveaux de poloxamère et de nanofiltration. L'invention concerne également des niveaux faibles de flux amélioré de poloxamère et des taux supérieurs retenus d'un facteur de croissance.
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