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1. WO2022023584 - METHODS OF INCREASING RECOMBINANT PROTEIN YIELDS

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Publication Number WO/2022/023584
Publication Date 03.02.2022
International Application No. PCT/EP2021/071596
International Filing Date 02.08.2021
IPC
C12N 1/14 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
C12P 21/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
C07K 14/38 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
37from fungi
38from Aspergillus
C12N 9/62 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50Proteinases
58derived from fungi
62from Aspergillus
C12N 1/16 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
CPC
C07K 14/38
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
37from fungi
38from Aspergillus
C12N 1/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
14Fungi
16Yeasts; Culture media therefor
Applicants
  • BIOTALYS NV [BE]/[BE]
Inventors
  • COCONI LINARES, Lucia Nancy
  • VAN POUCKE, Jolien
  • VERLINDE, Charlotte
Agents
  • LEONARD, Thomas
Priority Data
20188921.931.07.2020EP
20188933.431.07.2020EP
20217907.330.12.2020EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF INCREASING RECOMBINANT PROTEIN YIELDS
(FR) PROCÉDÉS D'AUGMENTATION DU RENDEMENT EN PROTÉINES RECOMBINANTES
Abstract
(EN) The present invention relates to methods for increasing yield of compounds of interest produced by microbial cells, in particular recombination proteins produced by microbial cells. The present invention also relates to the use of peptone as a yield increasing agent in a method of production of a compound of interest. The present invention provides the compounds of interest, such as recombinant proteins, obtained by the method of the invention.
(FR) La présente invention concerne des procédés visant à augmenter le rendement en composés d'intérêt produits par des cellules microbiennes, en particulier des protéines de recombinaison produites par des cellules microbiennes. La présente invention concerne également l'utilisation de peptone en tant qu'agent d'augmentation de rendement dans un procédé de production d'un composé d'intérêt. La présente invention concerne les composés d'intérêt, tels que des protéines recombinantes, obtenus par le procédé de l'invention.
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