Processing

Please wait...

Settings

Settings

Goto Application

1. WO2022023371 - INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING PARTIAL HARVEST

Publication Number WO/2022/023371
Publication Date 03.02.2022
International Application No. PCT/EP2021/071057
International Filing Date 27.07.2021
IPC
C12P 21/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
CPC
C12P 21/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
Applicants
  • BASF SE [DE]/[DE]
Inventors
  • DAUB, Andreas
  • GOLABGIR ANBARANI, Aydin
  • KLEIN, Tobias
  • MORWEISER, Michael
  • WANDREY, Georg
Agents
  • ALTMANN STÖSSEL DICK PATENTANWÄLTE PARTG MBB
Priority Data
20188161.228.07.2020EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING PARTIAL HARVEST
(FR) PROCÉDÉ DE FERMENTATION INDUSTRIELLE POUR BACILLUS UTILISANT UNE RÉCOLTE PARTIELLE
Abstract
(EN) The present invention relates to the field of industrial fermentation. In particular, it relates to method for producing a protein of interest from a Bacillus host cell comprising the steps of inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, cultivating the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest for a cultivation phase, wherein the cultivation of the Bacillus host cell during said cultivation phase comprises the addition of at least one feed solution and wherein the at least one feed solution provides a carbon source, and separating portions from said Bacillus host cell culture during the cultivation phase at different time points and obtaining the protein of interest from said portions.
(FR) La présente invention se rapporte au domaine de la fermentation industrielle. En particulier, elle concerne un procédé de production d'une protéine d'intérêt à partir d'une cellule hôte de Bacillus comprenant les étapes consistant à inoculer un milieu de fermentation avec une cellule hôte de Bacillus comprenant une construction d'expression pour un gène codant pour une protéine d'intérêt ; cultiver la cellule hôte de Bacillus dans ledit milieu de fermentation dans des conditions propices à la croissance de la cellule hôte de Bacillus et à l'expression de la protéine d'intérêt pour une phase de culture, la culture de la cellule hôte de Bacillus pendant ladite phase de culture comprenant l'ajout d'au moins une solution d'alimentation et la ou les solutions d'alimentation fournissant une source de carbone ; séparer des parties de ladite culture de cellules hôtes de Bacillus pendant la phase de culture à différents moments et obtenir la protéine d'intérêt à partir desdites parties.
Latest bibliographic data on file with the International Bureau