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1. WO2022011182 - METHODS AND COMPOSITIONS FOR ISOLATION AND RAPID DETECTION OF MICRO-ORGANISMS FROM BLOOD AND BODILY FLUIDS

Publication Number WO/2022/011182
Publication Date 13.01.2022
International Application No. PCT/US2021/040951
International Filing Date 08.07.2021
IPC
C12N 1/06 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
06Lysis of microorganisms
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
G01N 1/30 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
30Staining; Impregnating
Applicants
  • ACUTIS DIAGNOSTICS, INC. [US]/[US]
Inventors
  • SHANMUGAM, Mayilvahanan
  • AKHTAR, Mumtaz
  • GOLDBERG, David
  • BON HOMME, Marjorie
Agents
  • GREELEY, Paul, D.
Priority Data
63/050,50910.07.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND COMPOSITIONS FOR ISOLATION AND RAPID DETECTION OF MICRO-ORGANISMS FROM BLOOD AND BODILY FLUIDS
(FR) PROCÉDÉS ET COMPOSITIONS POUR L'ISOLEMENT ET LA DÉTECTION RAPIDE DE MICROORGANISMES À PARTIR DE SANG ET DE FLUIDES CORPORELS
Abstract
(EN) The present disclosure provides methods and compositions for testing blood samples to determine the presence and type of a blood stream infection (BSI). In one embodiment, the composition is a lysis reagent or composition that comprises betaine hydrochloride, spermidine, saponin, and Triton® X-100. The methods include combining the lysis reagent with the blood sample, and at least one centrifuge step to isolate the micro-organisms that cause the BSI. The micro-organisms are kept viable so that diagnostic tests can be run on the blood samples after the various method steps are performed.
(FR) La présente divulgation fournit des procédés et des compositions pour tester des échantillons de sang afin de déterminer la présence et le type d'une infection du sang (BSI). Dans un mode de réalisation, la composition est un réactif ou une composition de lyse qui comprend du chlorhydrate de bétaïne, de la spermidine, de la saponine et du Triton® X-100. Les procédés comprennent la combinaison du réactif de lyse avec l'échantillon de sang, et au moins une étape de centrifugation pour isoler les micro-organismes à l'origine de la BSI. Les micro-organismes sont conservés de manière viable de telle sorte que des tests de diagnostic peuvent être exécutés sur les échantillons de sang après que les différentes étapes du procédé sont effectuées.
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