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1. WO2022010662 - SEPARATING POLYNUCLEOTIDE FRAGMENTS

Publication Number WO/2022/010662
Publication Date 13.01.2022
International Application No. PCT/US2021/039019
International Filing Date 25.06.2021
IPC
C12Q 1/6806 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
Applicants
  • ILLUMINA, INC. [US]/[US]
  • ILLUMINA CAMBRIDGE LIMITED [GB]/[GB]
Inventors
  • BACIGALUPO, Maria Candelaria Rogert
  • STEEMERS, Frank
  • FISHER, Jeffrey
  • SLATTER, Andrew
  • KRAFT, Lewis
  • GORMLEY, Niall
  • BOWEN, M. Shane
Agents
  • SHEEHAN, Teige P.
Priority Data
63/048,34706.07.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SEPARATING POLYNUCLEOTIDE FRAGMENTS
(FR) SÉPARATION DE FRAGMENTS POLYNUCLÉOTIDIQUES
Abstract
(EN) Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.
(FR) L'invention concerne un procédé, comprenant l'étirement d'un polynucléotide sur un substrat comprenant une pluralité de régions de clivage espacées de manière égale comprenant une pluralité de transposases, le clivage du polynucléotide avec au moins deux transposases parmi la pluralité de transposases pour former une pluralité de fragments polynucléotidiques, et la séparation, au sein de la pluralité de fragments polynucléotidiques, d'une population de fragments polynucléotidiques plus longs à partir d'une population de fragments polynucléotidiques plus courts. L'invention concerne également un procédé comprenant l'étirement d'un polynucléotide sur un substrat comprenant une pluralité de régions de clivage espacées de manière égale comprenant une pluralité de transposases, le clivage du polynucléotide avec au moins deux transposases parmi la pluralité de transposases pour former une pluralité de fragments polynucléotidiques, et la séparation, au sein de la pluralité de fragments polynucléotidiques, d'une population de fragments polynucléotidiques plus longs à partir d'une population de fragments polynucléotidiques plus courts.
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