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1. WO2022009206 - SEX DETERMINATION MARKERS IN MULLETS AND METHODS OF USING THEREOF

Publication Number WO/2022/009206
Publication Date 13.01.2022
International Application No. PCT/IL2021/050834
International Filing Date 07.07.2021
IPC
C12Q 1/6879 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6879for sex determination
A01K 61/10 2017.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
61Culture of aquatic animals
10of fish
A01K 67/02 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67Rearing or breeding animals, not otherwise provided for; New breeds of animals
02Breeding vertebrates
Applicants
  • THE STATE OF ISRAEL, MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT, AGRICULTURAL RESEARCH ORGANIZATION (ARO) (VOLCANI INSTITUTE]) [IL]/[IL]
Inventors
  • RON, Michael
  • SHIRAK, Andrey
  • SEROUSSI, Eyal
  • ROSENFELD, Hanna
  • MEIRI ASHKENAZI, Iris
Agents
  • STERN, Johanna
Priority Data
27592608.07.2020IL
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SEX DETERMINATION MARKERS IN MULLETS AND METHODS OF USING THEREOF
(FR) MARQUEURS DE DÉTERMINATION DU SEXE DANS DES MULETS ET LEURS PROCÉDÉS D'UTILISATION
Abstract
(EN) The invention relates to a sex determination (SD) marker of a male Mugilidae fish comprising a nucleic acid sequence corresponding to a sequence of a Follicle-stimulating hormone receptor (FSHR) gene of said fish and including at least one male (Y) specific nucleotide difference when compared to a corresponding sequence on a FSHR gene of a female Mugilidae fish. The invention further concerns uses of this sex determination marker in methods of early sex determination in a Mugilidae fish, methods of sex-reversal of a Mugilidae fish prior to appearance of a sex phenotype, and methods for producing a mono-sex population of Mugilidae fishes.
(FR) L'invention concerne un marqueur de détermination du sexe (SD) d'un poisson Mugilidae mâle comprenant une séquence d'acide nucléique correspondant à une séquence d'un gène de récepteur d'hormone folliculo-stimulante (FSHR) dudit poisson et comprenant au moins une différence nucléotidique spécifique au mâle (Y) lorsqu'elle est comparée à une séquence correspondante sur un gène FSHR d'un poisson Mugilidae femelle. L'invention concerne également des utilisations de ce marqueur de détermination du sexe dans des procédés de détermination précoce du sexe chez un poisson Mugilidae, des procédés d'inversion du sexe d'un poisson Mugilidae avant l'apparition d'un phénotype sexuel, et des procédés de production d'une population mono-sexe de poissons Mugilidae.
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