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1. WO2022008498 - METHOD FOR THE DETECTION AND/OR QUANTIFICATION OF EXTRACELLULAR VESICLES IN FLUID BIOLOGICAL SAMPLES

Publication Number WO/2022/008498
Publication Date 13.01.2022
International Application No. PCT/EP2021/068633
International Filing Date 06.07.2021
IPC
G01N 33/50 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N 33/543 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
Applicants
  • CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC) [ES]/[ES]
  • UNIVERSIDAD AUTÓNOMA DE MADRID [ES]/[ES]
  • IMMUNOSTEP, S.L. [ES]/[ES]
Inventors
  • VALÉS GÓMEZ, María del Mar
  • CAMPOS SILVA, Carmen
  • CÁCERES MARTELL, Yaiza
  • REYBURN, Hugh Thomson
  • YÁÑEZ MÓ, María
  • JARA ACEBEDO, Ricardo
Agents
  • PONS ARIÑO, Angel
Priority Data
20382602.906.07.2020EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR THE DETECTION AND/OR QUANTIFICATION OF EXTRACELLULAR VESICLES IN FLUID BIOLOGICAL SAMPLES
(FR) MÉTHODE DE DÉTECTION ET/OU DE QUANTIFICATION DE VÉSICULES EXTRACELLULAIRES DANS DES ÉCHANTILLONS BIOLOGIQUES FLUIDES
Abstract
(EN) The invention relates to a method for detecting and/or quantifying extracellular vesicles (EVs) which are present in a biological fluid sample using a solid surface functionalized with a capture molecule, a detection molecule and polycationic polymers. The method of the invention is useful for the detection and/or quantification of biomarkers present in the surface of the EVs as well as in the diagnostic of diseases.
(FR) L'invention se rapporte à une méthode de détection et/ou de quantification de vésicules extracellulaires (EV) présentes dans un échantillon de fluide biologique à l'aide d'une surface solide fonctionnalisée à l'aide d'une molécule de capture, d'une molécule de détection et de polymères polycationiques. La méthode de l'invention est utile pour la détection et/ou la quantification de biomarqueurs présents dans la surface des EV ainsi que pour le diagnostic de maladies.
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