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1. WO2022007881 - MODIFIED GLUTAMATE DEHYDROGENASE AND USE THEREOF

Publication Number WO/2022/007881
Publication Date 13.01.2022
International Application No. PCT/CN2021/105179
International Filing Date 08.07.2021
IPC
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
06acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0012acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
0014acting on the CH-NH2 group of donors (1.4)
0016with NAD or NADP as acceptor (1.4.1)
Applicants
  • 湖南利尔生物科技有限公司 HUNAN LIER BIOTECHNOLOGY CO., LTD. [CN]/[CN]
Inventors
  • 谢新开 XIE, Xinkai
  • 徐伟 XU, Wei
  • 范俊英 FAN, Junying
Agents
  • 北京永新同创知识产权代理有限公司 NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD.
Priority Data
202010659106.809.07.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) MODIFIED GLUTAMATE DEHYDROGENASE AND USE THEREOF
(FR) GLUTAMATE DÉSHYDROGÉNASE MODIFIÉE ET SON UTILISATION
(ZH) 经修饰的谷氨酸脱氢酶及其应用
Abstract
(EN) Provided is modified glutamate dehydrogenase (GluDH). In particular, the modified GluDH provided has an improved activity of catalyzing PPO to react with an amino donor to generate L-glufosinate-ammonium and/or has an improved kinetic property. Further provided are a polynucleotide for encoding the modified GluDH, a vector and host cell for expressing the modified GluDH of the present invention, and a method for producing L-glufosinate-ammonium by means of using the modified GluDH and the host cell.
(FR) L'invention concerne une glutamate déshydrogénase (GluDH) modifiée. En particulier, la GluDH modifiée présente une activité améliorée de catalyse de PPO pour réagir avec un donneur d'amine afin de générer du L-glufosinate-ammonium et/ou présente une propriété cinétique améliorée. L'invention concerne en outre un polynucléotide pour le codage de la GluDH modifiée, un vecteur et une cellule hôte pour exprimer la GluDH modifiée de la présente invention, et un procédé de production de L-glufosinate-ammonium au moyen de la GluDH modifiée et de la cellule hôte.
(ZH) 提供经修饰的谷氨酸脱氢酶(GluDH)。具体而言,提供经修饰的GluDH具有提高的催化PPO与氨基供体反应生成L-草铵膦的活性和/或改进的动力学性质。还提供编码所述经修饰的GluDH的多核苷酸、表达本发明的经修饰的GluDH的载体和宿主细胞以及使用所述经修饰的GluDH和宿主细胞生产L-草铵膦的方法。
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