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1. WO2022007205 - METHOD FOR INCREASING CONTENT OF INTRACELLULAR HEME IN ESCHERICHIA COLI

Publication Number WO/2022/007205
Publication Date 13.01.2022
International Application No. PCT/CN2020/118317
International Filing Date 28.09.2020
IPC
C12N 1/21 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/31 2006.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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31Genes encoding microbial proteins, e.g. enterotoxins
C12N 15/53 2006.1
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52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
C12N 15/54 2006.1
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52Genes encoding for enzymes or proenzymes
54Transferases (2)
C12N 15/90 2006.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
C12N 15/70 2006.1
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
Applicants
  • 江南大学 JIANGNAN UNIVERSITY [CN]/[CN]
Inventors
  • 唐蕾 TANG, Lei
  • 潘梅 PAN, Mei
  • 刘爽欣 LIU, Shuangxin
Agents
  • 哈尔滨市阳光惠远知识产权代理有限公司 HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD.
Priority Data
202010651132.608.07.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR INCREASING CONTENT OF INTRACELLULAR HEME IN ESCHERICHIA COLI
(FR) PROCÉDÉ D'AUGMENTATION DE LA TENEUR EN HÈME INTRACELLULAIRE DANS ESCHERICHIA COLI
(ZH) 一种提高大肠杆菌胞内血红素含量的方法
Abstract
(EN) A method for increasing the content of intracellular heme in escherichia coli, comprising: knocking out a gene mscS for encoding a small-conductivity mechanosensitive ion channel protein, knocking out a gene aroG for encoding 3-deoxy-arabinoheptulose-7 phosphate synthase, or overexpressing a gene hemA for encoding glutamyl-tRNA reductase in escherichia coli. The recombinant bacteria obtained by construction are cultured in an LB culture medium, and the content of the heme can reach 47.6 µmol·L-1.
(FR) Procédé d'augmentation de la teneur en hème intracellulaire dans Escherichia coli, comprenant : l'invalidation d'un gène mscS pour coder une protéine de canal ionique mécanosensible à faible conductivité, l'invalidation d'un gène aroG pour le codage de la 3-désoxy-arabinoheptulose-7 phosphate synthase ou la surexpression d'un gène hemA pour le codage de la glutamyl-ARNt réductase dans Escherichia coli. Les bactéries recombinées obtenues par construction sont cultivées dans un milieu de culture LB et la teneur de l'hème peut atteindre 47,6 µmol·L-1.
(ZH) 一种提高大肠杆菌胞内血红素含量的方法,在大肠杆菌中敲除编码小电导类机械敏感型离子通道蛋白的基因mscS、敲除编码3-脱氧-阿拉伯庚酮糖-7磷酸合成酶的基因aroG,或过表达了编码谷氨酰-tRNA还原酶的基因hemA。构建得到的重组菌在LB培养基中进行培养,血红素含量可达47.6μmol·L -1
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