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1. WO2022006764 - RECOMBINANT DIAPHORINA CITRI CHITINASE-LIKE PROTEIN EN03, AND CODING GENE AND APPLICATION THEREOF

Publication Number WO/2022/006764
Publication Date 13.01.2022
International Application No. PCT/CN2020/100775
International Filing Date 08.07.2020
IPC
C12N 9/42 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
24acting on glycosyl compounds (3.2)
42acting on beta-1, 4-glucosidic bonds, e.g. cellulase
C12N 15/11 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12N 15/113 2010.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/56 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
55Hydrolases (3)
56acting on glycosyl compounds (3.2), e.g. amylase, galactosidase, lysozyme
A01N 63/00 2020.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
63Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
A01P 7/00 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
7Arthropodicides
Applicants
  • 赣南师范大学 GANNAN NORMAL UNIVERSITY [CN]/[CN]
Inventors
  • 余海中 YU, Haizhong
  • 卢占军 LU, Zhanjun
  • 谢妍馨 XIE, Yanxin
  • 李宁艳 LI, Ningyan
  • 喻修道 YU, Xiudao
  • 黄爱军 HUANG, Aijun
  • 易龙 YI, Long
  • 苏华楠 SU, Huanan
  • 钟八莲 ZHONG, Balian
Agents
  • 北京高沃律师事务所 BEIJING GAOWO LAW FIRM
Priority Data
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) RECOMBINANT DIAPHORINA CITRI CHITINASE-LIKE PROTEIN EN03, AND CODING GENE AND APPLICATION THEREOF
(FR) PROTÉINE EN03 RECOMBINÉE DE TYPE CHITINASE DE DIAPHORINA CITRI, GÈNE CODANT ET APPLICATION ASSOCIÉE
(ZH) 柑橘木虱几丁质结合蛋白Chitinase-like EN03及其编码基因和应用
Abstract
(EN) The present invention relates to the technical field of pest control, and provides recombinant Diaphorina citri chitinase-like protein EN03. The amino acid sequence of the recombinant Diaphorina citri chitinase-like protein EN03 is shown in SEQ ID NO.1. In the present invention, genes encoding the recombinant Diaphorina citri chitinase-like protein EN03 are silenced by means of RNA interference, thereby causing death of the Diaphorina citri due to incomplete wing molting or unsuccessful molting, thus achieving the effect of Diaphorina citri control.
(FR) La présente invention concerne le domaine technique de la lutte antiparasitaire, et propose la protéine EN03 recombinée de type chitinase de Diaphorina citri. La séquence d'acides aminés de la protéine recombinée EN03 de type chitinase de Diaphorina citri est illustrée à la SEQ ID NO : 1. Dans la présente invention, les gènes codant pour la protéine recombinée EN03 de type chitinase de Diaphorina citri sont réduits au silence par interférence ARN, provoquant ainsi la mort de Diaphorina citri en raison d'une mue incomplète des ailes ou d'une mue infructueuse, ce qui permet d'obtenir l'effet de lutte contre Diaphorina citri.
(ZH) 本发明提供了柑橘木虱几丁质结合蛋白Chitinase-like EN03,属于病虫害防治技术领域;所述柑橘木虱几丁质结合蛋白Chitinase-like EN03的氨基酸序列如SEQ ID NO.1所示。本发明通过RNA干扰沉默柑橘木虱几丁质结合蛋白Chitinase-like EN03的编码基因,能够使柑橘木虱翅蜕皮不完全或者蜕皮不成功导致死亡,从而达到防治柑橘木虱的效果。
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