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1. WO2022006260 - METHODS OF PREPARING SAMPLES FOR PROTEOMIC ANALYSIS

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Publication Number WO/2022/006260
Publication Date 06.01.2022
International Application No. PCT/US2021/039870
International Filing Date 30.06.2021
IPC
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/49 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
49blood
G01N 30/88 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
88Integrated analysis systems specially adapted therefor, not covered by a single one of groups G01N30/04-G01N30/86146
CPC
G01N 2570/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2570Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
G01N 33/6842
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
G01N 33/6848
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
Applicants
  • STRECK, INC. [US]/[US]
Inventors
  • SOBANSKY, Matthew, R.
  • GEORGE, Nicholas, Michael
  • HUNSLEY, Bradford, A.
  • SALAMIFAR, Sean
  • LENAGH, Agnes, Alicia
Agents
  • BRASHEAR, Jeanne, M.
Priority Data
63/047,14301.07.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF PREPARING SAMPLES FOR PROTEOMIC ANALYSIS
(FR) PROCÉDÉS DE PRÉPARATION D'ÉCHANTILLONS POUR ANALYSE PROTÉOMIQUE
Abstract
(EN) Provided herein are methods of preparing a protein sample for proteomic analysis. In exemplary embodiments, the method comprises (a) contacting a blood sample comprising proteins with a protective agent comprising an anticoagulant (AC) and an aldehyde releaser (AR), to obtain a mixture, optionally, wherein the blood sample is added to a blood collection tube (BCT) comprising the protective agent, and (b) isolating a fraction comprising proteins or a source of proteins from the mixture to yield a protein sample or a source of a protein sample, wherein steps of the method are carried out in the absence of exogenous proteolytic enzyme inhibitors, wherein the protein sample is suitable for proteomic and peptidomedic analysis.
(FR) La présente invention concerne des méthodes de préparation d'un échantillon de protéine pour une analyse protéomique. Dans des modes de réalisation donnés à titre d'exemple, la méthode comprend (a) la mise en contact d'un échantillon sanguin comprenant des protéines avec un agent de protection comprenant un anticoagulant (AC) et un agent de libération d'aldéhyde (AR), pour obtenir un mélange, facultativement, l'échantillon sanguin étant ajouté à un tube de collecte de sang (BCT) comprenant l'agent de protection, et (b) l'isolement d'une fraction comprenant des protéines ou une source de protéines à partir du mélange pour obtenir un échantillon de protéines ou une source d'un échantillon de protéines, les étapes de la méthode étant réalisées en l'absence d'inhibiteurs d'enzymes protéolytiques exogènes, l'échantillon de protéines étant approprié pour une analyse protéomique et peptidomique.
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