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1. WO2022006203 - SYNTHETIC ETHYLENE-RESPONSIVE BINDING ELEMENT AND USES THEREOF

Publication Number WO/2022/006203
Publication Date 06.01.2022
International Application No. PCT/US2021/039766
International Filing Date 30.06.2021
IPC
C12N 15/82 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
Applicants
  • NORTH CAROLINA STATE UNIVERSITY [US]/[US]
  • INSTITUTE OF CYTOLOGY AND GENETICS, SIBERIAN BRANCH OF RUSSIAN ACADEMY OF SCIENCES [RU]/[RU]
Inventors
  • STEPANOVA, Anna N.
  • ALONSO, Jose M.
  • FERNANDEZ-MORENO, Josefina Patricia
  • ZEMLYANSKAYA, Elena
  • LEVITSKY, Victor
Agents
  • MURPHY, Sherry L.
Priority Data
62/705,51601.07.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SYNTHETIC ETHYLENE-RESPONSIVE BINDING ELEMENT AND USES THEREOF
(FR) ÉLÉMENT DE LIAISON SYNTHÉTIQUE SENSIBLE À L'ÉTHYLÈNE ET SES UTILISATIONS
Abstract
(EN) Provided is a recombinant nucleic acid construct comprising a sequence of Formula I (SEQ ID NO:48): X-N1-N2-N3-N4-N5-N6-N7-N8-N9-N10-N11-Y as an ethylene binding sequence (EBS). Tandems of two or more directly adjoining EBS are also described, and may be included in an expression cassette useful for modulating the expression of a nucleic acid of interest in a plant in response to plant hormone ethylene.
(FR) L'invention concerne une construction d'acide nucléique recombinant Comprenant Une séquence De Formule I (SEQ ID NO : 48) : X-N 1-N 2-N 3-N 4-N 5-N 6 -N 7-N 8-N 9-N 10-N 11-Y en tant que séquence de liaison à l'éthylène (EBS) L'invention Concerne également des Tandems de deux EBS directement adjacentes ou plus, et peut être inclus dans une cassette d'expression utile pour moduler l'expression d'un acide nucléique d'intérêt dans une plante en réponse à l'éthylène-hormone végétale.
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