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1. WO2022004953 - GENETICALLY ENGINEERED YEAST HAVING ACETOIN-PRODUCING ABILITY AND METHOD FOR PRODUCING ACETOIN USING SAME

Publication Number WO/2022/004953
Publication Date 06.01.2022
International Application No. PCT/KR2020/014539
International Filing Date 22.10.2020
IPC
C12N 15/81 2006.1
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
C12N 9/10 2006.1
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
C12N 9/88 2006.1
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88Lyases (4.)
C12N 9/02 2006.1
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
C12P 7/26 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
CPC
C12N 15/81
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
C12N 9/0004
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
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C12N 9/88
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9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
C12P 7/26
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
Applicants
  • 서울대학교산학협력단 SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION [KR]/[KR]
Inventors
  • 한지숙 HAHN, Ji Sook
  • 배상정 BAE, Sang Jeong
Agents
  • 제일특허법인(유) FIRSTLAW P.C.
Priority Data
10-2020-008236403.07.2020KR
Publication Language Korean (ko)
Filing Language Korean (KO)
Designated States
Title
(EN) GENETICALLY ENGINEERED YEAST HAVING ACETOIN-PRODUCING ABILITY AND METHOD FOR PRODUCING ACETOIN USING SAME
(FR) LEVURE GÉNÉTIQUEMENT MODIFIÉE AYANT UNE CAPACITÉ DE PRODUCTION D'ACÉTOÏNE ET PROCÉDÉ DE PRODUCTION D'ACÉTOÏNE L'UTILISANT
(KO) 아세토인 생산능을 갖는 유전적으로 조작된 효모 및 이를 이용한 아세토인 생산방법
Abstract
(EN) The present invention relates to genetically engineered yeast having acetoin-producing ability and a method for producing acetoin using same. The genetically engineered yeast having acetoin-producing ability and yeast having excellent acetoin-producing ability according to the present invention have i) the ADH(alcohol dehydrogenase) gene, GPD(glycerol-3-phosphate dehydrogenase) gene, and BDH(2,3-butanediol) dehydrogenase) gene deleted therefrom, ii) the alsS gene, alsD gene and noxE gene introduced therein, and iii) mutated EMP46, PEP7, SUR1, and HXK2. When the yeast is used for producing acetoin, a greater amount of acetoin can be produced from the same amount of glucose. Therefore, the genetically engineered yeast and evolved yeast having acetoin-producing ability according to the present invention can be effectively used to produce acetoin in high yield.
(FR) La présente invention concerne une levure génétiquement modifiée ayant une capacité de production d'acétoïne et un procédé de production d'acétoïne l'utilisant. La levure génétiquement modifiée ayant une capacité de production d'acétoïne et une levure ayant une excellente capacité de production d'acétoïne selon la présente invention comportent i) une suppression du gène ADH (alcool déshydrogénase), du gène GPD(glycérol-3-phosphate déshydrogénase) et du gène BDH(2,3-butanediol déshydrogénase), ii) un ajout du gène alsS, du gène alsD et du gène noxE ajoutés à celles-ci et iii) EMP46, PEP7, SUR1 et HXK2 à l'état muté. Lorsque la levure est utilisée pour produire de l'acétoïne, une quantité supérieure d'acétoïne peut être produite à partir de la même quantité de glucose. Par conséquent, la levure génétiquement modifiée et la levure évoluée ayant une capacité de production d'acétoïne selon la présente invention peuvent être utilisées efficacement pour produire de l'acétoïne avec un rendement élevé.
(KO) 본 발명은 아세토인 생산능을 갖는 유전적으로 조작된 효모 및 이를 이용한 아세토인의 생산방법에 관한 것이다. 본 발명에 따른 아세토인 생산능을 갖는 유전적으로 조작된 효모 및 아세토인 생산능이 우수한 효모는 i) ADH(alcohol dehydrogenase) 유전자, GPD(glycerol-3-phosphate dehydrogenase) 유전자 및 BDH(2,3-butanediol dehydrogenase) 유전자가 결실되고, ii) alsS 유전자, alsD 유전자 및 noxE 유전자가 도입되며, iii) EMP46, PEP7, SUR1 및 HXK2가 돌연변이된 것으로서, 이를 이용하여 아세토인을 생산할 경우, 같은 양의 글루코스로부터 많은 양의 아세토인을 생산할 수 있다. 따라서, 본 발명에 따른 아세토인 생산능을 갖는 유전적으로 조작된 효모 및 진화된 효모는 아세토인을 고수율로 생산하는데 유용하게 사용될 수 있다.
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