Processing

Please wait...

PATENTSCOPE will be unavailable a few hours for maintenance reason on Tuesday 25.01.2022 at 9:00 AM CET
Settings

Settings

Goto Application

1. WO2022003184 - ACTIVATED REPORTER PROTEIN FOR THE DETECTION OF INFECTION IN A BIOLOGICAL SAMPLE

Publication Number WO/2022/003184
Publication Date 06.01.2022
International Application No. PCT/EP2021/068402
International Filing Date 02.07.2021
IPC
C07K 14/435 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
C12N 9/50 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50Proteinases
C12Q 1/37 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
34involving hydrolase
37involving peptidase or proteinase
Applicants
  • INSTITUT PASTEUR [FR]/[FR]
  • CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - CNRS [FR]/[FR]
Inventors
  • MEYER, Björn
  • VIGNUZZI, Marco
Agents
  • ERNEST GUTMANN - YVES PLASSERAUD SAS
Priority Data
20305758.303.07.2020EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) ACTIVATED REPORTER PROTEIN FOR THE DETECTION OF INFECTION IN A BIOLOGICAL SAMPLE
(FR) PROTÉINE RAPPORTEUR ACTIVÉE POUR LA DÉTECTION D'UNE INFECTION DANS UN ÉCHANTILLON BIOLOGIQUE
Abstract
(EN) The invention relates to novel means and processes for the detection of a virus in a biological sample comprising cells infected by the virus. In particular, the invention relates to a fluorescent reporter protein designed as a recombinant inactive form of flipGFP suitable for specific activation by viral components in particular by viral proteins, such as viral protease, wherein the viral component recognizes a cleavage site inserted in the recombinant flipGFP. The fluorescent reporter protein is suitable for use in an in vitro method of detection of virus infection in a biological sample when the virus is related to the viral components activating the inactive form of flipGFP into an active fluorescent flipGFP in a biological sample, especially a sample comprising cells, in particular unaltered cells.
(FR) L'invention concerne de nouveaux moyens et méthodes de détection d'un virus dans un échantillon biologique comprenant des cellules infectées par le virus. En particulier, l'invention concerne une protéine rapporteur fluorescente conçue sous une forme inactive recombinante de FlipGFP appropriée pour une activation spécifique par des constituants viraux, en particulier par des protéines virales, telle qu'une protéase virale, le constituant viral reconnaissant un site de clivage inséré dans la FlipGFP recombinante. La protéine rapporteur fluorescente est appropriée pour être utilisée dans une méthode in vitro de détection d'une infection virale dans un échantillon biologique lorsque le virus est lié aux constituants viraux activant la forme inactive de FlipGFP en une FlipGFP fluorescente active dans un échantillon biologique, en particulier un échantillon comprenant des cellules, en particulier des cellules non modifiées.
Related patent documents
Latest bibliographic data on file with the International Bureau