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1. WO2022002327 - SYSTEM AND METHOD FOR DETERMINING ENZYME ACTIVITY IN GRAIN MATERIAL

Publication Number WO/2022/002327
Publication Date 06.01.2022
International Application No. PCT/DK2021/050209
International Filing Date 25.06.2021
IPC
C12Q 1/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
G01N 21/27 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
27using photo-electric detection
G01N 21/75 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
G01N 33/573 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
573for enzymes or isoenzymes
CPC
C12Q 1/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
C12Q 1/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
40involving amylase
G01N 21/78
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
78producing a change of colour
Applicants
  • GLYCOSPOT APS [DK]/[DK]
Inventors
  • SCHÜCKEL, Julia
  • BAYER, Robert
  • PARÉS VIADER, Raimon
  • HANSEN, Lennart
  • KRACUN, Stjepan Krešimir
  • LACENTRA, Roberto Thomas
  • CHRISTENSEN, Troels Balmer
Agents
  • PLOUGMANN VINGTOFT A/S
Priority Data
PA 2020 0079803.07.2020DK
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SYSTEM AND METHOD FOR DETERMINING ENZYME ACTIVITY IN GRAIN MATERIAL
(FR) SYSTÈME ET PROCÉDÉ POUR DÉTERMINER L'ACTIVITÉ ENZYMATIQUE D'UN MATÉRIAU GRANULAIRE
Abstract
(EN) A method of determining activity of a target enzyme of a grain material is described. The method comprises providing a fluid extract sample of the grain material using a preselected extraction procedure, providing a dyed and/or chromogenic substrate for the target enzyme, subjecting the fluid extract sample to the substrate for a preselected incubating time, and determining the target enzyme activity of the grain material. The extraction time is relatively short while still obtaining high accuracy.
(FR) L'invention concerne un procédé de détermination de l'activité d'une enzyme cible d'un matériau granulaire. Le procédé consiste à utiliser un échantillon d'extrait de fluide du matériau granulaire à l'aide d'une procédure d'extraction présélectionnée, utiliser un substrat coloré et/ou chromogène pour l'enzyme cible, mettre en contact l'échantillon d'extrait de fluide avec le substrat pendant un temps d'incubation présélectionné, et déterminer l'activité enzymatique cible du matériau granulaire. Le temps d'extraction est relativement court tout en permettant d'obtenir une grande précision.
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