Processing

Please wait...

PATENTSCOPE will be unavailable a few hours for maintenance reason on Tuesday 25.01.2022 at 9:00 AM CET
Settings

Settings

Goto Application

1. WO2022001825 - KIT FOR DETECTING E-CADHERIN EXPRESSION OF PERIPHERAL BLOOD CIRCULATING TUMOR CELLS OF PANCREATIC CANCER PATIENT AND DETECTION METHOD

Publication Number WO/2022/001825
Publication Date 06.01.2022
International Application No. PCT/CN2021/102098
International Filing Date 24.06.2021
IPC
G01N 33/58 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
G01N 33/574 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
G01N 33/569 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
B01D 29/05 2006.1
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
29Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/-B01D27/170;   Filtering elements therefor
01with flat filtering elements
05supported
Applicants
  • 天津市肿瘤医院(天津医科大学肿瘤医院) TIANJIN MEDICAL UNIVERSITY CANCER INSTITUTE & HOSPITAL [CN]/[CN]
Inventors
  • 郝继辉 HAO, Jihui
  • 李胜 LI, Sheng
  • 任贺 REN, He
Agents
  • 济南泉城专利商标事务所 JINAN SPRING PATENT & TRADEMARK OFFICE
Priority Data
202010626819.401.07.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) KIT FOR DETECTING E-CADHERIN EXPRESSION OF PERIPHERAL BLOOD CIRCULATING TUMOR CELLS OF PANCREATIC CANCER PATIENT AND DETECTION METHOD
(FR) KIT POUR DÉTECTER L'EXPRESSION DE E-CADHÉRINE DE CELLULES TUMORALES CIRCULANTES DANS LE SANG PÉRIPHÉRIQUE D'UN PATIENT ATTEINT D'UN CANCER DU PANCRÉAS ET MÉTHODE DE DÉTECTION
(ZH) 一种检测胰腺癌患者外周血循环肿瘤细胞E-Cadherin表达的试剂盒及检测方法
Abstract
(EN) A kit for detecting E-Cadherin expression of peripheral blood circulating tumor cells of a pancreatic cancer patient and a detection method, belonging to the technical field of molecular biology. The kit comprises 45 mL of a diluent, 1 mL of a decolorizing solution, 0.5 mL of a staining solution A, 1 mL of a staining solution B, 100 μL of a mouse anti-human E-Cadherin primary antibody, 100 μL of a rabbit anti-mouse IgG/HRP linked to horse radish peroxidase, 100 μL of 0.1% Triton X-100, 100 μL of 0.3% H2O2, 15 mL of a reagent A, 50 mL of a reagent B and 60 mL of a 6*PBS buffer solution. According to the detection method, the CTC in peripheral blood of a patient with advanced or recurrent pancreatic cancer, which cannot obtain a tissue specimen, is separated by using a membrane filtration device, and the E-Cadherin expression condition of the CTC is further detected by using an immunohistochemical technology.
(FR) L'invention concerne un kit permettant de détecter l'expression de E-cadhérine de cellules tumorales circulantes (CTC) dans le sang périphérique d'un patient atteint d'un cancer du pancréas et une méthode de détection, appartenant au domaine technique de la biologie moléculaire. Le kit comprend 45 mL d'un diluant, 1 mL d'une solution de décoloration, 0,5 mL d'une solution de coloration A, 1 mL d'une solution de coloration B, 100 μL d'un anticorps primaire anti-E-cadhérine humain de souris, 100 μL d'un anti-IgG/HRP de souris de lapin lié à une peroxydase de raifort, 100 μL de Triton X-100 à 0,1 %, 100 μL de H2O2 à 0,3 %, 15 mL d'un réactif A, 50 mL d'un réactif B et 60 mL d'une solution tampon 6*PBS. Selon la méthode de détection, la CTC dans le sang périphérique d'un patient atteint d'un cancer pancréatique avancé ou récurrent, dont on ne peut pas obtenir un échantillon de tissu, est séparée à l'aide d'un dispositif de filtration à membrane, et la condition d'expression de E-cadhérine de la CTC est en outre détectée à l'aide d'une technologie immunohistochimique.
(ZH) 一种检测胰腺癌患者外周血循环肿瘤细胞E-Cadherin表达的试剂盒及检测方法,属于分子生物学技术领域。试剂盒,包括稀释液45mL、脱色液1mL、染色液A 0.5mL、染色液B 1mL、鼠抗人E-Cadherin一抗100μL、连接有辣根过氧化物酶的兔抗鼠IgG/HRP 100μL、0.1%Triton X-100 100μL、0.3%H 2O 2100μL、试剂A 15mL、试剂B 50mL、6×PBS缓冲液60mL。检测方法利用膜过滤装置分离获得无法获取组织标本的晚期或复发胰腺癌患者外周血中的CTC,进一步运用免疫组化技术检测CTC的E-Cadherin表达情况。
Related patent documents
Latest bibliographic data on file with the International Bureau