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1. WO2022001631 - DEVICE FOR PREPARING CELL CLUSTERS, CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF

Publication Number WO/2022/001631
Publication Date 06.01.2022
International Application No. PCT/CN2021/099718
International Filing Date 11.06.2021
IPC
C12M 3/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
CPC
C08J 2383/04
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G
2383Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen, or carbon only; Derivatives of such polymers
04Polysiloxanes
C08J 2389/00
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G
2389Characterised by the use of proteins; Derivatives thereof
C08J 2483/04
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G
2483Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen, or carbon only; Derivatives of such polymers
04Polysiloxanes
C08J 2489/00
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G
2489Characterised by the use of proteins; Derivatives thereof
C08J 3/24
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G
3Processes of treating or compounding macromolecular substances
24Crosslinking, e.g. vulcanising, of macromolecules
C08J 5/18
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G
5Manufacture of articles or shaped materials containing macromolecular substances
18Manufacture of films or sheets
Applicants
  • 清华大学 TSINGHUA UNIVERSITY [CN]/[CN]
Inventors
  • 姚睿 YAO, Rui
  • 苏奕君 SU, Yijun
  • 梁少君 LIANG, Shaojun
  • 薛胜楠 XUE, Shengnan
  • 申函宁 SHEN, Hanning
Agents
  • 北京路浩知识产权代理有限公司 CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED
Priority Data
202010610263.X29.06.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) DEVICE FOR PREPARING CELL CLUSTERS, CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF
(FR) DISPOSITIF DE PRÉPARATION DE GRAPPES DE CELLULES, PROCÉDÉ DE CONSTRUCTION CORRESPONDANT ET APPLICATION ASSOCIÉE
(ZH) 用于制备细胞团簇的装置及其构建方法及应用
Abstract
(EN) The present application provides a device for preparing cell clusters, a construction method therefor and an application thereof. The device comprises a cell culture plate, and a substrate that is fixed in a well of the cell culture plate and has a microcavity array. Each microcavity forms a space for cell cluster growth, and may also have a non-vertical wall surface. Cell cluster properties are obtained by means of shape and volume regulation. The preparation method therefor comprises: preparing a size-adjustable microcavity template, and then obtaining a substrate having a microcavity array by means of two-step rollover. The device provided by the present application can be used for preparing cell clusters in a large scale conveniently, and can be used repeatedly. The elastic modulus of the substrate can be adjusted to meet the requirements of co-culture of various types of cells and multiple types of cells. The cell clusters prepared using this device have high activity, uniform size, complete shape, controllable performance, and good biological properties, and can be used in cell therapy, high-throughput model construction, multi-cell co-culture, stem cell differentiation, organoid construction, drug screening, tissue regeneration, and in vitro artificial systems.
(FR) La présente invention concerne un dispositif de préparation de grappes de cellules, un procédé de construction correspondant et une application associée. Le dispositif comprend une plaque de culture cellulaire, et un substrat fixé dans un puits de la plaque de culture cellulaire et comportant un réseau de microcavités. Chaque microcavité forme un espace pour la croissance de la grappe de cellules, et peut également avoir une surface de paroi non verticale. Les propriétés des grappes de cellules sont obtenues par la régulation de la forme et du volume. Le procédé de préparation associé comprend les étapes suivantes : préparation d'un gabarit de microcavité à taille ajustable, puis obtention d'un substrat comportant un réseau de microcavités au moyen d'un retournement en deux étapes. Le dispositif selon la présente invention peut être utilisé pour préparer des grappes de cellules à grande échelle de manière commode, et peut être utilisé de manière répétée. Le module élastique du substrat peut être ajusté pour satisfaire aux exigences de co-culture de divers types de cellules et de multiples types cellulaires. Les grappes de cellules préparées à l'aide de ce dispositif présentent une activité élevée, une taille uniforme, une forme complète, des performances maîtrisables et de bonnes propriétés biologiques. Elles peuvent être utilisées pour la thérapie cellulaire, la construction de modèles à haut rendement, la co-culture multi-cellulaire, la différenciation des cellules souches, la construction d'organoïdes, le criblage de médicaments, la régénération des tissus et les systèmes artificiels in vitro.
(ZH) 本申请提供一种用于制备细胞团簇的装置及其构建方法及应用。该装置包括细胞培养板和固定于细胞培养板的孔内且具有微腔阵列的基底。每个微腔形成细胞团簇生长的空间,还可具备非垂直壁面,通过形状和体积调控获得的细胞团簇性质。其制备方法包括:制备尺寸可调的微腔模板,再通过两步翻模得到具有微腔阵列的基底。本申请提供的装置可以便捷、大规模制备细胞团簇,可重复使用;可调节基底的弹性模量,以达到各种细胞、多种细胞共培养的要求。采用本装置制备的细胞团簇活性高、大小均一、形状完整、性能可控、生物学性能良好,可用于细胞治疗、高通量模型构建、多细胞共培养、干细胞分化、类器官构建、药物筛选、组织再生和体外人工系统等方面。
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