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1. WO2021247949 - METHODS OF IDENTIFYING MARKERS OF GRAFT REJECTION

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Publication Number WO/2021/247949
Publication Date 09.12.2021
International Application No. PCT/US2021/035817
International Filing Date 04.06.2021
IPC
C12Q 1/6811 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6811Selection methods for production or design of target specific oligonucleotides or binding molecules
C12Q 1/6827 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6827for detection of mutation or polymorphism
C12Q 1/6876 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Applicants
  • SIRONA GENOMICS, INC. [US]/[US]
Inventors
  • KRISHNAKUMAR, Sujatha
  • LI, Ming
  • PALSGAARD, Peggy
  • MINDRINOS, Michael N.
Agents
  • BUCK, Todd
  • HOLMES-SON, Michelle
  • PETERS, Hannah
  • PINO, Mark
  • VARSHNEYA, Pooja
Priority Data
63/035,47705.06.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF IDENTIFYING MARKERS OF GRAFT REJECTION
(FR) MÉTHODES D'IDENTIFICATION DE MARQUEURS DE REJET DE GREFFE
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Abstract
(EN) The invention relates to polynucleotide probes, with each polynucleotide probe comprising two perfectly complementary strands. In some embodiments, each one of the strands comprises, in a 5' to 3' direction, a) a first target hybridization sequence, b) a first digital tag sequence, c) a first Halo barcode sequence, d) a first Halo amplification primer sequence, e) a reverse second Halo amplification primer sequence, f) a reverse second Halo barcode sequence, g) a reverse second digital tag sequence, and h) a reverse second target hybridization sequence. The invention also relates to methods of using these novel probes in to determine the levels of a minor population of DNA amongst a mixture of DNA from two different sources.
(FR) L'invention concerne des sondes polynucléotidiques, chaque sonde polynucléotidique comprenant deux brins parfaitement complémentaires. Dans certains modes de réalisation, chacun des brins comprend, dans une direction de 5' à 3', a) une première séquence d'hybridation cible, b) une première séquence d'étiquettes numériques, c) une première séquence de code-barres de Halo, d) une première séquence d'amorces d'amplification de Halo, e) une seconde séquence d'amorce d'amplification de Halo inverse, f) une seconde séquence de code-barres de Halo inverse, g) une seconde séquence d'étiquettes numériques inverse, et h) une seconde séquence d'hybridation de cible inverse. L'invention concerne également des méthodes d'utilisation de ces nouvelles sondes pour déterminer les niveaux d'une population mineure d'ADN parmi un mélange d'ADN provenant de deux sources différentes.
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