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1. WO2021242741 - METHOD AND SYSTEM FOR QUANTITATIVE THREE DIMENSIONAL MEASUREMENT OF DENSITY, ANISOTROPY, AND ORIENTATION WITHOUT LABEL

Publication Number WO/2021/242741
Publication Date 02.12.2021
International Application No. PCT/US2021/034028
International Filing Date 25.05.2021
IPC
G01N 21/21 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
21Polarisation-affecting properties
G01N 21/64 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G02B 21/26 2006.1
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
24Base structure
26Stages; Adjusting means therefor
G01J 4/00 2006.1
GPHYSICS
01MEASURING; TESTING
JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
4Measuring polarisation of light
G06T 7/00 2017.1
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
7Image analysis
CPC
G01N 21/21
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
21Polarisation-affecting properties
G01N 21/4795
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
47Scattering, i.e. diffuse reflection
4795spatially resolved investigating of object in scattering medium
G02B 21/0092
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
0092Polarisation microscopes
G02B 21/367
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
36arranged for photographic purposes or projection purposes
365Control or image processing arrangements for digital or video microscopes
367providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
G02B 27/283
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
27Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
28for polarising
283used for beam splitting or combining
G02B 5/201
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
5Optical elements other than lenses
20Filters
201in the form of arrays
Applicants
  • CHAN ZUCKERBERG BIOHUB, INC. [US]/[US]
Inventors
  • MEHTA, Shalin
  • YEH, Li-Hao
  • IVANOV, Ivan
Agents
  • LARGENT, Craig C.
  • APPLE, Randolph T.
Priority Data
63/030,84127.05.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND SYSTEM FOR QUANTITATIVE THREE DIMENSIONAL MEASUREMENT OF DENSITY, ANISOTROPY, AND ORIENTATION WITHOUT LABEL
(FR) PROCÉDÉ ET SYSTÈME DE MESURE QUANTITATIVE EN TROIS DIMENSIONS DE DENSITÉ, D'ANISOTROPIE ET D'ORIENTATION SANS ÉTIQUETTE
Abstract
(EN) A method of measuring optical properties of a specimen, for example, a uniaxial specimen, includes generating a plurality of illumination patterns incident on the specimen and, for each of the plurality of illumination patterns, collecting sample light passing through the specimen and detecting the collected sample light using a polarization state analyzer to form a set of polarization channels. The method also includes receiving a calibration tensor, converting the set of polarization channels for each of the illumination patterns into Stokes parameter maps using the calibration tensor, and deconvolving the Stokes parameter maps to provide volumetric measurement of permittivity tensor of the specimen, specifically, absorption, optical path length, optical anisotropy, and 3D orientation of the specimen.
(FR) Procédé de mesure de propriétés optiques d'un échantillon, par exemple, un échantillon uniaxial, consistant à générer une pluralité de motifs d'éclairage incidents sur l'échantillon et, pour chaque motif de la pluralité de motifs d'éclairage, collecter une lumière d'échantillon traversant l'échantillon et détecter la lumière d'échantillon collectée à l'aide d'un analyseur d'état de polarisation pour former un ensemble de canaux de polarisation. Le procédé consiste également à recevoir un tenseur d'étalonnage, à convertir l'ensemble de canaux de polarisation pour chacun des motifs d'éclairage en cartes de paramètres de Stokes à l'aide du tenseur d'étalonnage, et à déconvolutionner les cartes de paramètres de Stokes pour fournir une mesure volumétrique de tenseur de permittivité de l'échantillon, en particulier l'absorption, la longueur de trajet optique, l'anisotropie optique et l'orientation 3D de l'échantillon.
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