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1. WO2021231477 - GENERATING NUCLEIC ACIDS WITH MODIFIED BASES USING RECOMBINANT TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE

Publication Number WO/2021/231477
Publication Date 18.11.2021
International Application No. PCT/US2021/031843
International Filing Date 11.05.2021
IPC
C12N 9/1264
CPC
C07K 2319/35
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
35containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
C12N 9/1264
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1241Nucleotidyltransferases (2.7.7)
1264DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applicants
  • ILLUMINA, INC. [US]/[US]
  • ILLUMINA SINGAPORE PTE. LTD. [SG]/[SG]
Inventors
  • NIRANTAR, Saurabh Rajendra
  • PEISAJOVICH, Sergio
  • BRUSTAD, Eric
Agents
  • CHANG, Y. Paul
Priority Data
63/023,73612.05.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) GENERATING NUCLEIC ACIDS WITH MODIFIED BASES USING RECOMBINANT TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE
(FR) GÉNÉRATION D'ACIDES NUCLÉIQUES AVEC DES BASES MODIFIÉES AU MOYEN DE DÉSOXYNUCLÉOTIDYL TRANSFÉRASE TERMINALE RECOMBINANTE
Abstract
(EN) Disclosed herein include methods of generating a single stranded deoxyribonucleic acid (ssDNA) scaffold comprising nucleotides with modified bases using a recombinant terminal deoxynucleotidyl transferase (TdT). The recombinant TdT can comprise an amino acid sequence that is at least 80% identical to a Bos taurus TdT, or a fragment thereof, and for example comprise one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His420 in the Bos taurus TdT.
(FR) L'invention concerne des procédés de génération d'un échafaudage d'acide désoxyribonucléique simple brin (ADNsb) comprenant des nucléotides avec des bases modifiées au moyen d'une désoxynucléotidyl transférase terminale recombinante (TdT). La transférase TdT recombinante peut comprendre une séquence d'acides aminés qui est au moins 80 % identique à une transférase TdT Bos taurus, ou un fragment de celle-ci, et comprendre par exemple une ou plusieurs mutations de substitution d'acide aminé en une ou plusieurs positions fonctionnellement équivalentes à Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, et His420 dans la transférase TdT Bos taurus.
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