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1. WO2021123847 - FLUORESCENCE METHOD FOR ASSESSING TRANSPORT ACTIVITY OF OATPS AND MRP2

Publication Number WO/2021/123847
Publication Date 24.06.2021
International Application No. PCT/HU2020/050014
International Filing Date 27.04.2020
IPC
G01N 33/50 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 33/5008
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
5008for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
G01N 33/6872
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6872Intracellular protein regulatory factors and their receptors, e.g. including ion channels
Applicants
  • CELLPHARMA KFT [HU]/[HU]
Inventors
  • LACZKA, Csilla
  • SZÉKELY, Virág
  • BAKOS, Éva
  • PATIK, Izabel
Agents
  • DANUBIA SZABADALMI ÉS JOGI IRODA KFT.
Priority Data
HU190044820.12.2019HU
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) FLUORESCENCE METHOD FOR ASSESSING TRANSPORT ACTIVITY OF OATPS AND MRP2
(FR) PROCÉDÉ DE FLUORESCENCE PERMETTANT L'ÉVALUATION DE L'ACTIVITÉ DE TRANSPORT D'OATP ET DE MRP2
Abstract
(EN) The invention relates to the field of assessing transport activity of OATP (Organic anion-transporting polypeptide) and ABC (ATP Binding Cassette) transport proteins. In particular, the invention relates to methods for determining in a single, fluorescence-based assay whether a compound is a modulator of OATP and/or MRP2 (Multidrug resistance associated protein, ABCC) transport activity, using a fluorescent compound that is a substrate of both OATP and MRP2, and is selected from sulfopyrenes having general formula (I) or sulforhodamines having general formula (II).
(FR) L'invention se rapporte au domaine de l'évaluation de l'activité de transport des OATP (polypeptide de transport d'anions organiques) et de protéines de transport ABC (cassette liant l'ATP). En particulier, l'invention se rapporte à des procédés qui permettent de déterminer, dans un dosage unique fondé sur la fluorescence, si un composé constitue un modulateur d'activité de transport d'OATP et/ou de MRP2 (protéine associée à une résistance multiple aux médicaments, ABCC), à l'aide d'un composé fluorescent qui constitue un substrat à la fois d'OATP et de MRP2, et qui est choisi parmi les sulfopyrenes ayant pour formule générale (I) ou les sulforhodamines ayant pour formule générale (II).
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