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1. WO2021090594 - NEUTROPHIL PROGENITOR CELLS AND METHOD FOR PRODUCING SAME

Publication Number WO/2021/090594
Publication Date 14.05.2021
International Application No. PCT/JP2020/036094
International Filing Date 24.09.2020
IPC
A61P 7/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
7Drugs for disorders of the blood or the extracellular fluid
C12N 5/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
A61K 35/15 2015.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
14Blood; Artificial blood
15Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
Applicants
  • 国立大学法人 東京大学 THE UNIVERSITY OF TOKYO [JP]/[JP]
Inventors
  • 宮内 将 MIYAUCHI, Masashi
  • 黒川 峰夫 KUROKAWA, Mineo
  • 伊藤 雄介 ITO, Yusuke
Agents
  • 青木 篤 AOKI, Atsushi
  • 三橋 真二 MITSUHASHI, Shinji
  • 渡辺 陽一 WATANABE, Yoichi
  • 中島 勝 NAKAJIMA, Masaru
  • 武居 良太郎 TAKEI, Ryotaro
  • 津田 英直 TSUDA, Hidenao
Priority Data
62/929,99104.11.2019US
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) NEUTROPHIL PROGENITOR CELLS AND METHOD FOR PRODUCING SAME
(FR) CELLULES PROGÉNITRICES DES NEUTROPHILES ET LEUR PROCÉDÉ DE PRODUCTION
(JA) 好中球前駆細胞及びその製造方法
Abstract
(EN)
A purpose of the present invention is to produce neutrophil-like cells in numbers that can be used in granulocyte transfusion therapy. It became possible to vastly improve proliferation by further forcibly expressing at least one gene belonging to the BCL2 family in neutrophil progenitor cells in which an Myc-C gene and a BMI1 gene are forcibly expressed. As a result, neutrophil progenitor cells having proliferation ability for more than 12 weeks after induction of differentiation from stem cells and capable of proliferating to a cell count of 1013 or higher were produced, leading to the present invention. Therefore, the present invention relates to such neutrophil progenitor cells, and a method for producing the same. The present invention also relates to neutrophil-like cells induced to differentiate from proliferated neutrophil progenitor cells, and a method for producing the same.
(FR)
La présente invention concerne la production de cellules type neutrophile en nombres qui peuvent être utilisées dans la thérapie de transfusion de granulocytes. Il devient possible d'améliorer largement la prolifération en exprimant de manière forcée et supplémentaire au moins un gène appartenant à la famille BCL2 dans les cellules progénitrices des neutrophiles dans lesquelles un gène Myc-C et un gène BMI1 sont exprimés de force. En résultat, les cellules progénitrices des neutrophiles présentant une capacité de prolifération durant plus de 12 semaines après l'induction de la différenciation à partir des cellules souches et étant susceptibles de proliférer jusqu'à un nombre de cellules produites de 1013 ou plus, conduisant à l'invention. Par conséquent, la présente invention concerne de telles cellules progénitrices des neutrophiles, et un procédé pour les produire. La présente invention concerne également des cellules type neutrophile induites pour se différencier à partir de cellules progénitrices des neutrophiles proliférées, et un procédé pour les produire.
(JA)
本発明は、顆粒球輸血療法に使用しうる数の好中球様細胞を製造することを目的とする。Myc-C遺伝子及びBMI1遺伝子が強制発現された好中球前駆細胞において、さらにBCL2ファミリーに属する少なくとも1の遺伝子を強制発現させることにより、増殖性を大幅に改善することが可能になった。これにより、幹細胞から分化誘導後に12週を超えて増殖能を有し、1013個以上の細胞数への増殖可能である好中球前駆細胞を製造し、本発明にいたった。したがって、本発明はかかる好中球前駆細胞、及びその製造方法に関する。さらに、本発明は増殖された好中球前駆細胞から分化誘導された好中球様細胞、及びその製造方法にも関する。
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