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1. WO2021088370 - HIGHLY EFFICIENT AND CONTROLLABLE EXPRESSION SYSTEM FOR ENDOGENOUS PIGGYBACKING EXOGENEOUS GENES

Publication Number WO/2021/088370
Publication Date 14.05.2021
International Application No. PCT/CN2020/096010
International Filing Date 14.06.2020
IPC
C12N 15/113 2010.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 15/81 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
C12N 1/19 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
19modified by introduction of foreign genetic material
C12R 1/865 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
645Fungi
85Saccharomyces
865Saccharomyces cerevisiae
CPC
C12N 15/113
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
C12N 15/81
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
C12N 2830/36
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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2830Vector systems having a special element relevant for transcription
36being a transcription termination element
Applicants
  • 中国农业科学院生物技术研究所 BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES [CN]/[CN]
Inventors
  • 徐玉泉 XU, Yuquan
  • 岳群 YUE, Qun
  • 张礼文 ZHANG, Liwen
Agents
  • 北京知本村知识产权代理事务所(普通合伙) BEIJING ZHIBENCUN INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP)
Priority Data
201911072118.405.11.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) HIGHLY EFFICIENT AND CONTROLLABLE EXPRESSION SYSTEM FOR ENDOGENOUS PIGGYBACKING EXOGENEOUS GENES
(FR) SYSTÈME D'EXPRESSION HAUTEMENT EFFICACE ET RÉGLABLE POUR GÈNES EXOGÈNES SUPERPOSÉS ENDOGÈNES
(ZH) 一种内源搭载的外源基因高效可控表达系统
Abstract
(EN)
Provided is a fungal operon, an exogeneous gene site-directed quantitative fixed-time expression system based on the fungal operon, and applications of the system in expressing heterogeneous genes. The system comprises the following sequences connected in series: a host target gene stop codon upstream fragment containing a stop codon, a fungal operon intergenic region, a target gene, and a DNA fragment of a host target gene stop codon downstream fragment. The system utilizes the fungal operon intergenic region to serially connect the target gene, inserts same to the downstream of a host cell expression gene stop codon by means of site-directed gene insertion, and utilizes a promoter of the target gene to complete the transcriptional expression of the target gene.
(FR)
L'invention concerne un opéron fongique, un système d'expression en temps fixe quantitatif dirigé sur site génique exogène basé sur l'opéron fongique, et des applications du système dans l'expression de gènes hétérogènes. Le système comprend les séquences suivantes reliées en série : un fragment amont de codon d'arrêt de gène cible hôte contenant un codon d'arrêt, une région intergénique d'opéron fongique, un gène cible, et un fragment d'ADN d'un fragment aval de codon d'arrêt de gène cible hôte. Le système utilise la région intergénique de l'opéron fongique pour connecter en série le gène cible, les insérer à l'aval d'un codon d'arrêt de gène d'expression de cellule hôte au moyen d'une insertion de gène dirigée sur site, et utilise un promoteur du gène cible pour achever l'expression transcriptionnelle du gène cible.
(ZH)
提供了一种真菌操纵子,以所述真菌操纵子为基础的外源基因定点定量定时表达系统及其在表达异源基因中的应用,所述系统包括顺序连接的以下序列:含终止密码子的宿主靶基因终止密码子上游片段、真菌操纵子基因间隔区、目标基因、宿主靶基因终止密码子下游片段的DNA片段。所述系统利用真菌操纵子基因间隔区序列连接目标基因,通过定点基因插入的方式将其插入到宿主细胞表达基因终止密码子的下游,利用靶基因的启动子完成目标基因的转录表达。
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