Processing

Please wait...

Settings

Settings

Goto Application

1. WO2021063355 - METHOD FOR CONSTRUCTING FOOD-GRADE ETHANOL-DEGRADING BACILLUS SUBTILIS RECOMBINANT BACTERIA

Publication Number WO/2021/063355
Publication Date 08.04.2021
International Application No. PCT/CN2020/118857
International Filing Date 29.09.2020
IPC
C12N 15/66 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C12N 15/75 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75for Bacillus
C12N 1/21 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
A61K 35/742 2015.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
66Microorganisms or materials therefrom
74Bacteria
741Probiotics
742Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
A61P 39/02 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
39General protective or antinoxious agents
02Antidotes
C12R 1/125 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
07Bacillus
125Bacillus subtilis
CPC
A61K 35/742
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
66Microorganisms or materials therefrom
74Bacteria
741Probiotics
742Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
A61P 39/02
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
39General protective or antinoxious agents
02Antidotes
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
20Bacteria
C12N 15/66
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C12N 15/75
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75for Bacillus
Applicants
  • 南京农业大学 NANJING AGRICULTURAL UNIVERSITY [CN]/[CN]
  • 南京福斯弗瑞生物科技有限公司 NANJING FOODSAFETY BIOTECH CO., LTD. [CN]/[CN]
Inventors
  • 陆兆新 LU, Zhaoxin
  • 卢静 LU, Jing
  • 李金良 LI, Jinliang
  • 吕凤霞 LV, Fengxia
  • 张充 ZHANG, Chong
Agents
  • 南京经纬专利商标代理有限公司 NANJING JINGWEI PATENT & TRADEMARK AGENCY CO., LTD
Priority Data
201910940788.730.09.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR CONSTRUCTING FOOD-GRADE ETHANOL-DEGRADING BACILLUS SUBTILIS RECOMBINANT BACTERIA
(FR) PROCÉDÉ DE CONSTRUCTION DE BACTÉRIES RECOMBINANTES DE BACILLUS SUBTILIS DÉGRADANT L'ÉTHANOL DE QUALITÉ ALIMENTAIRE
(ZH) 一种食品级降解乙醇枯草杆菌重组菌的构建方法
Abstract
(EN)
Provided is a method for constructing food-grade ethanol-degrading Bacillus subtilis recombinant bacteria. The method integrates expression cassettes of two enzyme genes into the genome of Bacillus subtilis by means of double-crossover homologous recombination, thus realizing the intracellular co-expression of ethanol dehydrogenase and acetaldehyde dehydrogenase. Recombinant Bacillus subtilis can degrade ethanol under low pH conditions, which can be converted into acetic acid under the action of ethanol dehydrogenase and acetaldehyde dehydrogenase.
(FR)
L'invention concerne un procédé de construction de bactéries recombinantes de Bacillus subtilis dégradant l'éthanol de qualité alimentaire. Le procédé intègre des cassettes d'expression de deux gènes enzymatiques dans le génome de Bacillus subtilis au moyen d'une recombinaison homologue par enjambement double, ce qui permet de réaliser la co-expression intracellulaire de l'éthanol déshydrogénase et de l'acétaldéhyde déshydrogénase. Bacillus subtilis recombinant peut dégrader l'éthanol dans des conditions de pH bas, qui peut être converti en acide acétique sous l'action de l'éthanol déshydrogénase et de l'acétaldéhyde déshydrogénase.
(ZH)
提供一种食品级降解乙醇的枯草杆菌重组菌的构建方法。该方法通过双交换同源重组的方法将两个酶基因的表达盒整合至枯草杆菌的基因组上,实现了乙醇脱氢酶和乙醛脱氢酶的胞内共表达。重组枯草杆菌可以在低pH条件下降解乙醇,经乙醇脱氢酶和乙醛脱氢酶的作用下可以转化为乙酸。
Also published as
Latest bibliographic data on file with the International Bureau