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1. WO2021058713 - CONCENTRATED PERFUSION MEDIUM

Publication Number WO/2021/058713
Publication Date 01.04.2021
International Application No. PCT/EP2020/076836
International Filing Date 25.09.2020
IPC
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
CPC
C12N 5/0018
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0018Culture media for cell or tissue culture
C12N 5/0031
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0018Culture media for cell or tissue culture
0031Serum-free culture media
C12N 5/005
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0018Culture media for cell or tissue culture
005Protein-free medium
Applicants
  • BOEHRINGER INGELHEIM INTERNATIONAL GMBH [DE]/[DE]
Inventors
  • COFFMAN, Jonathan
  • LIN, Henry
  • LUMAN, Todd
  • OGAWA, Daisie
  • RAVIKRISHNAN, Janani
  • TESSMAN, Hayden
  • YILDIRIM, Samet
  • YU, Marcella
Agents
  • SIMON, Elke Anna Maria
  • STOCK, Dr. Wolfgang
  • HONG, Dr. Michael
Priority Data
62/906,98327.09.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CONCENTRATED PERFUSION MEDIUM
(FR) MILIEU DE PERFUSION CONCENTRÉ
Abstract
(EN)
The invention relates to a serum-free cell culture perfusion medium comprising the medium components subgrouped into at least three separate aqueous concentrated feeds and a diluent, wherein the resulting serum-free cell culture perfusion medium is pH-adjusting to neutral pH upon mixing. Also provided is a method of preparing said serum-free cell culture perfusion medium. The invention further relates to methods of culturing mammalian cells or producing a protein of interest in perfusion culture using said serum-free cell culture perfusion medium that achieve high productivity at a low cell specific perfusion rate. The invention further relates to the use of the new and improved serum-free cell culture perfusion medium to control osmolality in a perfusion cell culture, wherein increasing osmolality results in an increase in total productivity and/or cell specific productivity by suppressing cell growth during cell culture, e.g., during production phase of perfusion cell culture. Suppression of cell growth particularly reduces or eliminates the need for wasteful cell bleed.
(FR)
L'invention concerne un milieu de perfusion de culture cellulaire sans sérum comprenant les composants du milieu sous-groupés en au moins trois charges concentrées aqueuses séparées et un diluant, le milieu de perfusion de culture cellulaire sans sérum résultant étant ajusté en pH à un pH neutre lors du mélange. L'invention concerne également un procédé de préparation dudit milieu de perfusion de culture cellulaire sans sérum. L'invention concerne en outre des procédés de culture de cellules de mammifère ou de production d'une protéine d'intérêt dans une culture de perfusion à l'aide dudit milieu de perfusion de culture cellulaire sans sérum qui permet d'obtenir une productivité élevée à un faible taux de perfusion spécifique à une cellule. L'invention concerne en outre l'utilisation du nouveau milieu de perfusion de culture cellulaire sans sérum amélioré pour réguler l'osmolalité dans une culture de cellules de perfusion, l'augmentation de l'osmolalité entraînant une augmentation de la productivité totale et/ou de la productivité spécifique des cellules par suppression de la croissance cellulaire pendant la culture cellulaire, par exemple, pendant la phase de production de la culture de cellules de perfusion. La suppression de la croissance cellulaire permet en particulier de réduire ou d'éliminer la nécessité d'un prélèvement de cellules inutiles.
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