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1. WO2021058115 - METHODS FOR THE BIOCATALYTICAL MANUFACTURING OF DIHYDROCHALCONES

Publication Number WO/2021/058115
Publication Date 01.04.2021
International Application No. PCT/EP2019/076265
International Filing Date 27.09.2019
IPC
C12P 7/26 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
A23L 27/30 2016.01
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D91; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
27Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
30Artificial sweetening agents
C12P 17/06 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
17Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms
02Oxygen as only ring hetero atoms
06containing a six-membered hetero ring, e.g. fluorescein
CPC
A23L 27/30
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT
27Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
30Artificial sweetening agents
C12P 17/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
17Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
02Oxygen as only ring hetero atoms
06containing a six-membered hetero ring, e.g. fluorescein
C12P 7/26
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
Applicants
  • SYMRISE AG [DE]/[DE]
Inventors
  • GEISSLER, Torsten
  • LEY, Jakob Peter
  • ZIRPEL, Bastian
Agents
  • EISENFÜHR SPEISER PATENTANWÄLTE RECHTSANWÄLTE PARTGMBB
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR THE BIOCATALYTICAL MANUFACTURING OF DIHYDROCHALCONES
(FR) PROCÉDÉS DE FABRICATION BIOCATALYTIQUE DE DIHYDROCHALCONES
Abstract
(EN)
The present invention relates to a biocatalytical method for manufacturing of homoeriodictyol dihydrochalcone and/or hesperetin dihydrochalcone by providing at least one first biocatalyst system for the hydroxylation of phloretin and/or its glycosides as well as at least one second biocatalyst for the methylation of 3-hydroxyphloretin. Further disclosed are microorganisms capable of producing such biocatalysts as well as sequences encoding the biocatalysts. Furthermore, the present invention relates to the use of a mixture obtained by a method as disclosed in the present invention and to specific compositions suitable as sweetness enhancers and/or flavouring agents.
(FR)
La présente invention concerne un procédé biocatalytique pour la fabrication d'homoériodictyol dihydrochalcone et/ou d'hespérétine dihydrochalcone en fournissant au moins un premier système biocatalyseur pour l'hydroxylation de phlorétine et/ou ses glycosides ainsi qu'au moins un second biocatalyseur pour la méthylation de la 3-hydroxyphlorétine. L'invention concerne en outre des micro-organismes capables de produire de tels biocatalyseurs ainsi que des séquences codant pour les biocatalyseurs. En outre, la présente invention concerne l'utilisation d'un mélange obtenu par un procédé selon la présente invention et des compositions spécifiques appropriées en tant qu'exhausteurs de sucrosité et/ou agents aromatisants.
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