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1. WO2021056120 - A PAPER-BASED MICROFLUIDIC DON-CHIP FOR RAPID AND LOW-COST DEOXYNIVALENOL QUANTIFICATION IN FOODS, FEEDS AND FEED INGREDIENTS

Publication Number WO/2021/056120
Publication Date 01.04.2021
International Application No. PCT/CA2020/051289
International Filing Date 28.09.2020
IPC
G01N 33/558 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
558using diffusion or migration of antigen or antibody
G01N 33/02 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
02Food
G01N 33/10 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
02Food
10Starch-containing substances, e.g. dough
CPC
B01L 2300/0636
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
06Auxiliary integrated devices, integrated components
0627Sensor or part of a sensor is integrated
0636Integrated biosensor, microarrays
B01L 2300/069
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
06Auxiliary integrated devices, integrated components
069Absorbents; Gels to retain a fluid
B01L 2300/0825
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0809rectangular shaped
0825Test strips
B01L 2300/126
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
12Specific details about materials
126Paper
B01L 2300/161
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
16Surface properties and coatings
161Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
B01L 2400/0406
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0406capillary forces
Applicants
  • UNIVERSITY OF MANITOBA [CA]/[CA]
Inventors
  • JIANG, Qian
  • WU, Jiandong
  • LIN, Francis
  • YANG, Chengbo
Agents
  • ADE & COMPANY INC.
Priority Data
62/906,44126.09.2019US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) A PAPER-BASED MICROFLUIDIC DON-CHIP FOR RAPID AND LOW-COST DEOXYNIVALENOL QUANTIFICATION IN FOODS, FEEDS AND FEED INGREDIENTS
(FR) PUCE À DÉSOXYNIVALÉNOL (PUCE-DON) MICROFLUIDIQUE À BASE DE PAPIER POUR QUANTIFICATION DE DÉSOXYNIVALÉNOL RAPIDE ET ÉCONOMIQUE DANS DE LA NOURRITURE, DES ALIMENTS ET DES INGRÉDIENTS D'ALIMENTS
Abstract
(EN) A rapid, low-cost, portable and reliable method for on-site detection of deoxynivalenol (DON), a representative mycotoxin predominantly occurring in grains, would be helpful to control mycotoxin contamination. Herein, a paper-based microfluidic chip capable of measuring deoxynivalenol (DON-Chip) in foods, feeds and feed ingredients was developed. As discussed herein, the DON-Chip incorporated a colorimetric competitive immunoassay into a paper microfluidic device and used gold nanoparticles as a signal indicator. Furthermore, a novel ratiometric analysis method was used to improve signal resolvability at low concentrations of DON. Detection of DON in aqueous extracts from solid foods, feeds or feed ingredients was successfully validated with a detection range from 0.01-20 ppm (using dilution factors from 10-104). Compared with conventional methods, the novel DON-Chip greatly reduces the cost and time of mycotoxin detection in the food and feed industry.
(FR) L'invention concerne un procédé rapide, économique, portable et fiable pour la détection sur site de désoxynivalénol (DON), une mycotoxine représentative survenant principalement dans des céréales, qui serait utile pour lutter contre une contamination par mycotoxines. Ainsi, une puce microfluidique à base de papier apte à mesurer le désoxynivalénol (puce-DON) dans de la nourriture, des aliments et des ingrédients d'aliments a été développée. Comme discuté ici, la puce-DON incorpore un dosage immunologique compétitif colorimétrique dans un dispositif microfluidique en papier et utilise des nanoparticules d'or en tant qu'indicateur de signal. En outre, un nouveau procédé d'analyse ratiométrique a été utilisé pour améliorer la capacité de résolution de signal à de faibles concentrations de DON. La détection de DON dans des extraits aqueux provenant de nourriture, d'aliments ou d'ingrédients d'aliments solides a été validée avec succès avec une plage de détection de 0,01 à 20 ppm (à l'aide de facteurs de dilution de 10 à 104). Comparativement aux procédés classiques, la nouvelle puce-DON réduit considérablement le coût et le temps de détection de mycotoxines dans l'industrie de la nourriture et des aliments.
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