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1. WO2021041322 - METHODS AND CONSTRUCTS FOR PRODUCTION OF LENTIVIRAL VECTOR

Publication Number WO/2021/041322
Publication Date 04.03.2021
International Application No. PCT/US2020/047650
International Filing Date 24.08.2020
Chapter 2 Demand Filed 25.09.2020
IPC
C12N 15/86 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 15/85 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 5/10 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 7/00 2006.01
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7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
C12N 15/62 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
CPC
C07K 14/161
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
08RNA viruses
15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
16HIV-1 ; ; HIV-2
161gag-pol, e.g. p55, p24/25, p17/18, p7, p6, p66/68, p51/52, p31/34, p32, p40
C07K 14/162
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07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
08RNA viruses
15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
16HIV-1 ; ; HIV-2
162env, e.g. gp160, gp110/120, gp41, V3, peptid T, CD4-Binding site
C07K 14/163
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07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
08RNA viruses
15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
16HIV-1 ; ; HIV-2
163Regulatory proteins, e.g. tat, nef, rev, vif, vpu, vpr, vpt, vpx
C12N 15/86
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2740/15051
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2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
15011Lentivirus, not HIV, e.g. FIV, SIV
15051Methods of production or purification of viral material
C12N 2740/16052
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2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
16011Human Immunodeficiency Virus, HIV
16051Methods of production or purification of viral material
16052relating to complementing cells and packaging systems for producing virus or viral particles
Applicants
  • LONZA WALKERSVILLE, INC. [US]/[US]
Inventors
  • SHIN, Young
  • SETH, Anandita
  • GU, Bingnan
Agents
  • MILLS, Jeffrey K.
Priority Data
62/890,90423.08.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND CONSTRUCTS FOR PRODUCTION OF LENTIVIRAL VECTOR
(FR) MÉTHODES ET CONSTRUCTIONS DE PRODUCTION DE VECTEUR LENTIVIRAL
Abstract
(EN)
The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.
(FR)
La présente invention concerne des méthodes de production de cellules de production de vecteur lentiviral. Plus particulièrement, les méthodes font appel à deux plasmides, au lieu de quatre, pour fournir les éléments d'encapsidation requis et pour transférer un vecteur vers une cellule, permettant la production d'un grand nombre de cellules productrices lentivirales, comprenant des cellules à base de suspension, et la production de grandes quantités de lentivirus. Ces méthodes permettent la production de cellules qui peuvent être induites plus tard pour produire un lentivirus, et peuvent être personnalisées pour comprendre un gène d'intérêt spécifique.
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