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1. WO2021037886 - METHODS FOR RAPID cDNA PRODUCTION AND CLONING

Publication Number WO/2021/037886
Publication Date 04.03.2021
International Application No. PCT/EP2020/073809
International Filing Date 26.08.2020
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6844 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
CPC
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/1096
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1096cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
C12Q 2521/107
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2521Reaction characterised by the enzymatic activity
10Nucleotidyl transfering
107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
C12Q 2531/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2531Reactions of nucleic acids characterised by
10the purpose being amplify/increase the copy number of target nucleic acid
Applicants
  • INSTITUTE FOR RESEARCH IN BIOMEDICINE [CH]/[CH]
Inventors
  • BARBIERI, Sonia
  • RAVASIO, Sara
Agents
  • VON STOSCH, Andreas
  • EICHELBAUM, Kornelia
  • LEHMBACHER, Michael
  • SCHARPING, Marion
Priority Data
PCT/EP2019/07311229.08.2019EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR RAPID cDNA PRODUCTION AND CLONING
(FR) PROCÉDÉS DE PRODUCTION ET DE CLONAGE D'ADNC RAPIDE
Abstract
(EN)
The present invention provides a method for rapid generation and cloning of a DNA molecule encoding a polypeptide of interest, such as an antibody. The method combines RACE for cDNA generation with circular polymerase extension cloning (CPEC) and is, thus, largely sequence independent. The present invention also provides methods for production and sequencing of a protein of interest, such as an antibody, as well as primers, vectors and cells useful in such methods.
(FR)
La présente invention concerne un procédé de production et de clonage rapides d'une molécule d'ADN codant pour un polypeptide d'intérêt, tel qu'un anticorps. Le procédé combine RACE pour la production d'ADNc avec un clonage d'extension de polymérase circulaire (CPEC) et est, ainsi, largement indépendant de la séquence. La présente invention concerne également des procédés de production et de séquençage d'une protéine d'intérêt, telle qu'un anticorps, ainsi que des amorces, des vecteurs et des cellules utiles dans de tels procédés.
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