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1. WO2021036995 - DIRECT MICRORNA SEQUENCING USING ENZYME ASSISTED SEQUENCING

Publication Number WO/2021/036995
Publication Date 04.03.2021
International Application No. PCT/CN2020/110836
International Filing Date 24.08.2020
IPC
C12Q 1/68 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 33/487 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
CPC
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
G01N 33/487
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
487of liquid biological material
Applicants
  • NANJING UNIVERSITY [CN]/[CN]
Inventors
  • HUANG, Shuo
  • ZHANG, Jinyue
  • YAN, Shuanghong
Agents
  • CHENG & PENG INTELLECTUAL PROPERTY LAW OFFICE
Priority Data
PCT/CN2019/10216223.08.2019CN
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DIRECT MICRORNA SEQUENCING USING ENZYME ASSISTED SEQUENCING
(FR) SÉQUENÇAGE DIRECT DE MICRO-ARN À L'AIDE D'UN SÉQUENÇAGE ASSISTÉ PAR ENZYME
Abstract
(EN)
Provided are a method of determining the sequence of a short RNA, and the seqencing library and system thereof. The method is performed by enzyme assisted sequencing, especially "Nanopore Induced Phase-Shift Sequencing (NIPSS)", using a DNA-RNA chimeric substrate, and can be used to sequence short RNA such as miRNA, siRNA or piRNA.
(FR)
L'invention concerne un procédé de détermination de la Séquence d'un ARN court, et la banque de séquences et son système. Le procédé est mis en oeuvre par séquençage assisté par enzyme, en particulier par séquençage par déphasage induit par nanopores (NIPSS), à L'aide d'un substrat chimérique ADN-ARN, et peut être utilisé pour séquencer un ARN court tel qu'un miARN, siARNsi ou piARN.
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