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1. WO2021027250 - DETECTION MATERIAL, PREPARATION METHOD AND APPLICATION OF ANTI-NMDAR AUTOANTIBODY IN HUMAN BODY FLUID

Publication Number WO/2021/027250
Publication Date 18.02.2021
International Application No. PCT/CN2020/071289
International Filing Date 10.01.2020
IPC
C07K 14/705 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
C07K 1/14 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
C12N 15/861 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
861Adenoviral vectors
G01N 33/531 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
G01N 33/564 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
564for pre-existing immune complex or autoimmune disease
CPC
C07K 14/70571
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
70571for neuromediators, e.g. serotonin receptor, dopamine receptor
C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2710/10343
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
10011Adenoviridae
10311Mastadenovirus, e.g. human or simian adenoviruses
10341Use of virus, viral particle or viral elements as a vector
10343viral genome or elements thereof as genetic vector
C12N 2800/107
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2800Nucleic acids vectors
10Plasmid DNA
106for vertebrates
107for mammalian
G01N 2333/70571
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
705Assays involving receptors, cell surface antigens or cell surface determinants
70571for neuromediators, e.g. serotonin receptor, dopamine receptor
G01N 2800/28
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
28Neurological disorders
Applicants
  • 陕西脉元生物科技有限公司 SHAANXI MYBIOTECH CO., LTD. [CN]/[CN]
Inventors
  • 闫亚平 YAN, Yaping
  • 李怡婷 LI, Yiting
  • 郝文斌 HAO, Wenbin
  • 李科 LI, Ke
Agents
  • 西安恒泰知识产权代理事务所 HENG TAI INTELLECTUAL PROPERTY AGENCY
Priority Data
201910738488.012.08.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) DETECTION MATERIAL, PREPARATION METHOD AND APPLICATION OF ANTI-NMDAR AUTOANTIBODY IN HUMAN BODY FLUID
(FR) MATÉRIAU DE DÉTECTION, PROCÉDÉ DE PRÉPARATION ET APPLICATION D'AUTO-ANTICORPS ANTI-NMDAR DANS UN FLUIDE CORPOREL HUMAIN
(ZH) 人体液中抗NMDAR自身抗体的检测材料、制备方法及应用
Abstract
(EN)
Provided are a detection material, a preparation method and an application of an anti-NMDAR autoantibody in human body fluid, NMDAR antigen is coated on a carrier film to prepare an anti-NMDAR receptor detection material, a specific NMDAR antibody in human serum and cerebrospinal fluid can bond with the antigen, an alkaline phosphatase substrate-ligand reaction is carried out for color development, a sealing material is added to the color reaction, and it can be directly judged whether the NMDAR antibody is contained in the detection sample by visual observation. The method has the advantages of high sensitivity, simple and convenient operation, rapid detection and the like, and is beneficial to the recognition and diagnosis of anti-NMDAR receptor encephalitis.
(FR)
L'invention concerne un matériau de détection, un procédé de préparation et une application d'un auto-anticorps anti-NMDAR dans un fluide corporel humain, l'antigène NMDAR est revêtu sur un film de support pour préparer un matériau de détection de récepteur anti-NMDAR, un anticorps anti-NMDAR spécifique dans le sérum et le liquide céphalorachidien humains peut se lier à l'antigène, une réaction substrat-ligand de phosphatase alcaline est réalisée pour un développement de couleur, un matériau d'étanchéité est ajouté à la réaction colorée, et il est possible de directement évaluer la présence de l'anticorps anti-NMDAR dans l'échantillon de détection par observation visuelle. Le procédé présente les avantages d'une sensibilité élevée, d'un fonctionnement simple et pratique, d'une détection rapide et équivalents, et est bénéfique pour la reconnaissance et le diagnostic de l'encéphalite à anticorps anti-NMDA.
(ZH)
提供人体液中抗NMDAR自身抗体的检测材料、制备方法及应用,将NMDAR抗原包被于载体膜上制成抗NMDAR受体检测材料,人血清和脑脊液中的特异性NMDAR抗体会与抗原结合,利用碱性磷酸酶底物-配体反应进行显色,并在显色反应中加入封闭材料,可用通过肉眼观察直接判断检测样品中是否含有NMDAR抗体。该方法具有灵敏度高,操作简便,快速检测等优点,有助于抗NMDAR受体脑炎的识别和诊断。
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