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1. WO2021027236 - METHOD FOR CONSTRUCTING DNA LIBRARY AND APPLICATION THEREOF

Publication Number WO/2021/027236
Publication Date 18.02.2021
International Application No. PCT/CN2019/130250
International Filing Date 31.12.2019
IPC
C12Q 1/6806 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C40B 50/06 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50Methods of creating libraries, e.g. combinatorial synthesis
06Biochemical methods, e.g. using enzymes or whole viable microorganisms
CPC
C12N 15/1093
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1093General methods of preparing gene libraries, not provided for in other subgroups
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C40B 50/06
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50Methods of creating libraries, e.g. combinatorial synthesis
06Biochemical methods, e.g. using enzymes or whole viable microorganisms
Applicants
  • 安诺优达基因科技(北京)有限公司 ANNOROAD GENE TECHNOLOGY (BEIJING) CO., LTD [CN]/[CN]
  • 浙江安诺优达生物科技有限公司 ZHEJIANG ANNOROAD BIO-TECHNOLOGY CO., LTD. [CN]/[CN]
Inventors
  • 陈晓丹 CHEN, Xiaodan
  • 徐护朝 XU, Huchao
  • 潘伟业 PAN, Weiye
  • 李志民 LI, Zhimin
  • 李大为 LI, Dawei
  • 玄兆伶 XUAN, Zhaoling
  • 王海良 WANG, Hailiang
  • 王娟 WANG, Juan
Priority Data
201910740285.512.08.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR CONSTRUCTING DNA LIBRARY AND APPLICATION THEREOF
(FR) PROCÉDÉ DE CONSTRUCTION D'UNE BIBLIOTHÈQUE D'ADN ET SON APPLICATION
(ZH) 构建DNA文库的方法及其应用
Abstract
(EN)
The present invention provides a method for constructing a DNA library and application thereof. The method comprises: providing chimeric DNA with a marker; performing transposition treatment on the chimeric DNA with a marker to obtain a transposition product; performing capture treatment on the transposition product to obtain captured DNA; and performing amplification treatment on the captured DNA to obtain the DNA library.
(FR)
La présente invention concerne un procédé de construction d'une bibliothèque d'ADN et son application. Le procédé comprend les étapes consistant à : fournir de l'ADN chimérique avec un marqueur ; effectuer un traitement de transposition sur l'ADN chimérique avec un marqueur pour obtenir un produit de transposition ; la mise en oeuvre d'un traitement de capture sur le produit de transposition pour obtenir de l'ADN capturé ; et la mise en oeuvre d'un traitement d'amplification sur l'ADN capturé pour obtenir la bibliothèque d'ADN.
(ZH)
本发明提供构建DNA文库的方法及其应用,所述方法包括:提供嵌合标记物的DNA;将所述嵌合标记物的DNA进行转座处理,得到转座产物;对所述转座产物进行捕获处理,得到捕获后的DNA;以及将所述捕获后的DNA进行扩增处理,获得所述DNA文库。
Also published as
Latest bibliographic data on file with the International Bureau