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1. WO2021011436 - SEPARATION AND QUANTIFICATION OF EMPTY AND FULL VIRAL CAPSID PARTICLES

Publication Number WO/2021/011436
Publication Date 21.01.2021
International Application No. PCT/US2020/041741
International Filing Date 12.07.2020
IPC
B01D 15/16 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08Selective adsorption, e.g. chromatography
10characterised by constructional or operational features
16relating to the conditioning of the fluid carrier
B01D 15/36 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
36involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
B01D 15/42 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08Selective adsorption, e.g. chromatography
42characterised by the development mode, e.g. by displacement or by elution
C12N 7/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
02Recovery or purification
CPC
B01D 15/166
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
10characterised by constructional or operational features
16relating to the conditioning of the fluid carrier
166Fluid composition conditioning, e.g. gradient
B01D 15/363
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
36involving ionic interaction
361Ion-exchange
363Anion-exchange
B01D 15/424
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
42characterised by the development mode, e.g. by displacement or by elution
424Elution mode
C12N 2750/14151
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2750ssDNA Viruses
00011ssDNA Viruses
14011Parvoviridae
14111Dependovirus, e.g. adenoassociated viruses
14151Methods of production or purification of viral material
C12N 7/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
Applicants
  • SANGAMO THERAPEUTICS, INC. [US]/[US]
Inventors
  • KHATWANI, Santoshkumar
  • PIROT, Zhu
Agents
  • LI, Z. Ying
  • LEE, Wyan-ching, M.
  • CHATTERJEE, Debashree
Priority Data
62/873,61912.07.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SEPARATION AND QUANTIFICATION OF EMPTY AND FULL VIRAL CAPSID PARTICLES
(FR) SÉPARATION ET QUANTIFICATION DE PARTICULES DE CAPSIDE VIRALE VIDES ET COMPLÈTES
Abstract
(EN)
The present disclosure provides methods for the separation and quantification of empty and full viral capsids (e.g., AAV capsids) within a viral preparation, such as a viral pharmaceutical composition or drug product.
(FR)
La présente invention concerne des procédés pour la séparation et la quantification de capsides virales vides et complets (par exemple, des capsides d'AAV) dans une préparation virale, telle qu'une composition pharmaceutique virale ou un produit médicamenteux.
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