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1. WO2021010369 - METHOD FOR DETECTING CIRCULATING TUMOR CELLS USING VIRUS

Publication Number WO/2021/010369
Publication Date 21.01.2021
International Application No. PCT/JP2020/027203
International Filing Date 13.07.2020
IPC
C12N 5/09 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
09Tumour cells
C12N 5/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 7/01 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
01Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
C12Q 1/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G01N 33/48 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
G01N 33/574 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
CPC
C12N 5/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
10Cells modified by introduction of foreign genetic material
C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G01N 33/48
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
G01N 33/574
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
Applicants
  • 学校法人順天堂 JUNTENDO EDUCATIONAL FOUNDATION [JP]/[JP]
  • オンコリスバイオファーマ株式会社 ONCOLYS BIOPHARMA INC. [JP]/[JP]
Inventors
  • 岡部 隆宏 OKABE Takahiro
  • 十合 晋作 TOGO Shinsaku
  • 高橋 和久 TAKAHASHI Kazuhisa
  • 阿部 可奈恵 ABE Kanae
  • 浦田 泰生 URATA Yasuo
Agents
  • 小林 浩 KOBAYASHI Hiroshi
  • 大森 規雄 OHMORI Norio
  • 植田 渉 UEDA Wataru
  • 鈴木 康仁 SUZUKI Yasuhito
Priority Data
2019-13041212.07.2019JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR DETECTING CIRCULATING TUMOR CELLS USING VIRUS
(FR) PROCÉDÉ DE DÉTECTION DE CELLULES TUMORALES CIRCULANTES À L'AIDE D'UN VIRUS
(JA) ウイルスを用いた循環腫瘍細胞の検出方法
Abstract
(EN)
[Problem] To provide a method for detecting circulating tumor cells. [Solution] This method for detecting a circulating tumor cell includes (a) a step for preparing a test sample by subjecting a blood sample to density-gradient centrifugation in the presence of a crosslinking agent for erythrocytes and leukocytes and thereby removing blood cells, (b) a step for infecting the test sample prepared in step (a) with a virus, (c) a step for labeling the test sample obtained in step (b), and (d) a step for detecting circulating tumor cells in the test sample obtained in step (c).
(FR)
Le problème décrit par la présente invention est de fournir un procédé de détection de cellules tumorales circulantes. La solution selon l'invention porte sur un procédé de détection d'une cellule tumorale circulante qui comprend (a) une étape de préparation d'un échantillon de test par soumission d'un échantillon de sang à une centrifugation à gradient de densité en présence d'un agent de réticulation pour les érythrocytes et les leucocytes, (b) une étape d'infection de l'échantillon de test préparé à l'étape (a) avec un virus, (c) une étape de marquage de l'échantillon de test obtenu à l'étape (b), et (d) une étape de détection de cellules tumorales circulantes dans l'échantillon de test obtenu à l'étape (c).
(JA)
【課題】循環腫瘍細胞を検出する方法の提供。 【解決手段】循環腫瘍細胞を検出する方法であって、以下の工程:(a)赤血球と白血球との架橋剤存在下で血液サンプルを密度勾配遠心処理して血球が除去された被検試料を調製する工程、(b)工程(a)で調製された被検試料に、ウイルスを感染させる工程、(c)工程(b)で得られた被検試料を標識する工程、及び(d)工程(c)で得られた被検試料から、循環腫瘍細胞を検出する工程を含む前記方法。
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