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1. WO2021008046 - SYSTEM AND METHOD FOR PREPARING MRNA

Publication Number WO/2021/008046
Publication Date 21.01.2021
International Application No. PCT/CN2019/119385
International Filing Date 19.11.2019
IPC
C12M 1/40 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
40Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
C12M 1/38 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
36including condition or time responsive control, e.g. automatically controlled fermentors
38Temperature-responsive control
C12M 1/36 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
36including condition or time responsive control, e.g. automatically controlled fermentors
C12M 1/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
02with agitation means; with heat exchange means
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
B01L 2200/141
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
14Process control and prevention of errors
141Preventing contamination, tampering
B01L 2300/1822
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
18Means for temperature control
1805Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
1822using Peltier elements
B01L 2300/1894
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
18Means for temperature control
1894Cooling means; Cryo cooling
B01L 2400/0666
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
06Valves, specific forms thereof
0633with moving parts
0666Solenoid valves
B01L 7/52
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7Heating or cooling apparatus
52with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
Applicants
  • 中国人民解放军军事科学院军事医学研究院 ACADEMY OF MILITARY MEDICAL SCIENCES [CN]/[CN]
Inventors
  • 王升启 WANG, Shengqi
  • 肖瑞 XIAO, Rui
  • 魏红娟 WEI, Hongjuan
  • 荣振 RONG, Zhen
  • 刘丽艳 LIU, Liyan
Agents
  • 北京超凡宏宇专利代理事务所(特殊普通合伙) CHOFN INTELLECTUAL PROPERTY
Priority Data
201910648409.717.07.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) SYSTEM AND METHOD FOR PREPARING MRNA
(FR) SYSTÈME ET PROCÉDÉ DE PRÉPARATION D'ARNM
(ZH) mRNA制备系统及制备方法
Abstract
(EN)
The present application belongs to the technical field of mRNA preparation, and discloses a system and a method for preparing mRNA, which can shorten a preparation period, and avoid degradation of an mRNA product due to the introduction of RNase by manual pipetting. The system for preparing mRNA comprises a PCR amplification device configured to amplify DNA; a plurality of raw reagent tubes, each of which is configured to provide a single reagent; and a reaction device configured to allow reagents to react. Specifically, the reaction device comprises a test tube rack configured to support reaction tubes, a semiconductor chilling plate connected to the test tube rack, a fin type radiator disposed under the test tube rack, and a cooling fan disposed under the fin type radiator; wherein the raw reagent tubes are connected to the reaction tubes via a solenoid valve module and a peristaltic pump module, and the solenoid valve module and the peristaltic pump module are connected with a control device and are controlled by an upper computer control program.
(FR)
La présente invention concerne un système et un procédé de préparation d'ARNm, pouvant raccourcir une période de préparation, et éviter la dégradation d'un produit d'ARNm du fait de l'introduction de RNase par pipetage manuel. Le système de préparation d'ARNm comprend un dispositif d'amplification par PCR configuré pour amplifier l'ADN ; une pluralité de tubes de réactif brut, dont chacun est configuré pour fournir un réactif unique ; et un dispositif de réaction configuré pour permettre à des réactifs de réagir. En particulier, le dispositif de réaction comprend un rack de tubes à essai configurée pour supporter des tubes de réaction, une plaque de refroidissement de semi-conducteur reliée au rack de tubes à essai, un radiateur de type à ailette disposé sous le rack de tubes à essai, et un ventilateur de refroidissement disposé sous le radiateur de type à ailette ; les tubes de réactif bruts étant reliés aux tubes de réaction par l'intermédiaire d'un module de vanne électromagnétique et d'un module de pompe péristaltique, et le module de vanne électromagnétique et le module de pompe péristaltique étant connectés à un dispositif de commande et étant commandés par un programme de commande d'ordinateur supérieur.
(ZH)
一种mRNA制备系统及制备方法,涉及mRNA制备技术领域,其能够缩短制备周期,同时避免因手工加液移液引入RNA酶而造成mRNA产物的降解。一种mRNA制备系统,包括:PCR扩增装置,配置成对DNA进行扩增;多个原料试剂管,每个原料试剂管配置成提供单一试剂;反应装置,配置成供试剂反应;具体地,反应装置包括:配置成承载反应管的试管架,与试管架连接的半导体制冷片,试管架的下方设置有翅片式散热器,翅片式散热器的下方设置有散热风扇;其中,原料试剂管与反应管之间通过电磁阀组件和蠕动泵组件连通,且电磁阀组件和蠕动泵组件连接有控制装置,并通过上位机控制程序进行控制。
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