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1. WO2021006286 - CELL CLUSTER CONTRACTION-RELAXATION MOVEMENT CHARACTERISTIC CALCULATION METHOD

Publication Number WO/2021/006286
Publication Date 14.01.2021
International Application No. PCT/JP2020/026636
International Filing Date 08.07.2020
IPC
C12M 1/00 2006.01
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MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
3Tissue, human, animal or plant cell, or virus culture apparatus
C12Q 1/04
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Applicants
  • 株式会社幹細胞&デバイス研究所 STEM CELL & DEVICE LABORATORY, INC. [JP]/[JP]
Inventors
  • 堀 晃輔 HORI Kosuke
  • 河原 紀之 KAWAHARA Noriyuki
  • 鳥羽 慶 TOBA Kei
Agents
  • 特許業務法人謝国際特許商標事務所 SHA PATENT AND TRADEMARK FIRM
Priority Data
2019-12805710.07.2019JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) CELL CLUSTER CONTRACTION-RELAXATION MOVEMENT CHARACTERISTIC CALCULATION METHOD
(FR) PROCÉDÉ DE CALCUL DE CARACTÉRISTIQUE DE MOUVEMENT DE CONTRACTION-RELAXATION D'ENSEMBLE DE CELLULES
(JA) 細胞塊収縮弛緩運動特性算出方法
Abstract
(EN)
[Problem] To provide a cell cluster contraction-relaxation movement characteristic calculation method that enables easy culturing of homogenized contractile cell clusters and easy calculation of contraction-relaxation movement characteristics of the cultured cell clusters. [Solution] This cell cluster contraction-relaxation movement characteristic calculation method involves: culturing of contractile cell clusters in replaceable cell holding devices 100; image-capturing, by using a cell cluster contraction-relaxation movement image, cell cluster contraction-relaxation movement information indicating the contraction-relaxation movement state of contractile cell clusters cultured in a cell culturing part 105 of a first cell holding device 100; and calculating, by using the acquired cell cluster contraction-relaxation movement information, contraction-relaxation movement characteristics of contractile cell clusters cultured in a cell culturing part 105 of a second cell holding device 100.
(FR)
La présente invention a pour but fournir un procédé de calcul de caractéristique de mouvement de contraction-relaxation d'ensemble de cellules permettant une culture facile d'ensembles de cellules contractiles homogénéisées et un calcul facile des caractéristiques de mouvement de contraction-relaxation des ensembles de cellules cultivées. La solution selon l'invention porte sur un procédé de calcul de caractéristique de mouvement de contraction-relaxation d'ensemble de cellules comprenant la culture d'ensembles de cellules contractiles dans des dispositifs de maintien de cellules remplaçables (100) ; la capture d'image, à l'aide d'une image de mouvement de contraction-relaxation d'ensemble de cellules, des informations de mouvement de contraction-relaxation d'ensemble de cellules indiquant l'état de mouvement de relaxation-relaxation d'ensembles de cellules contractiles cultivées dans une partie de culture de cellules (105) d'un premier dispositif de maintien de cellules (100); et calculer, à l'aide des informations de mouvement de contraction-relaxation d'ensemble de cellules acquises, des caractéristiques de mouvement de contraction-relaxation d'ensembles de cellules contractiles cultivées dans une partie de culture de cellules (105) d'un second dispositif de maintien de cellules (100).
(JA)
【課題】 均一化された収縮性細胞塊を容易に培養し、培養した細胞塊の収縮弛緩運動特性を容易に算出できる細胞塊収縮弛緩運動特性算出方法の提供。 【解決手段】 細胞塊収縮弛緩運動特性算出方法では、交換できる細胞保持装置100に収縮性細胞塊を培養し、第1の細胞保持装置100の細胞培養部105に培養された収縮性細胞塊の収縮弛緩運動状態を示す細胞塊収縮弛緩運動情報を、細胞塊収縮弛緩運動画像を用いて、画像取得し、取得した前記細胞塊収縮弛緩運動情報を用いて、第2の細胞保持装置100の細胞培養部105に培養された収縮性細胞塊の収縮弛緩運動特性を算出する。
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