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1. WO2021002630 - NOVEL PROMOTER HASP1 OF PHAEODACTYLUM TRICORNUTUM AND SIGNAL PEPTIDE THEREOF, AND USE THEREOF

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Publication Number WO/2021/002630
Publication Date 07.01.2021
International Application No. PCT/KR2020/008251
International Filing Date 25.06.2020
IPC
C12N 15/79 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
C12N 15/67 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12P 21/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
C07K 7/08 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
7Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
04Linear peptides containing only normal peptide links
08having 12 to 20 amino acids
Applicants
  • 한국과학기술연구원 KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY [KR]/[KR]
  • 주식회사 알지프로나 ALGAEPRONA INC. [KR]/[KR]
Inventors
  • 판철호 PAN, Cheol-Ho
  • 에르덴돌고르에르덴-오치르 ERDENEDOLGOR, Erdene-Ochir
  • 송대근 SONG, Dae-geun
  • 신복규 SHIN, Bok-Kyu
  • 권벼리 KWON, Byeori
Agents
  • 특허법인한얼 HANOL INTELLECTUAL PROPERTY AND LAW
Priority Data
10-2019-007901401.07.2019KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) NOVEL PROMOTER HASP1 OF PHAEODACTYLUM TRICORNUTUM AND SIGNAL PEPTIDE THEREOF, AND USE THEREOF
(FR) NOUVEAU PROMOTEUR HASP1 DE PHAEODACTYLUM TRICORNUTUM ET SON PEPTIDE SIGNAL, ET SON UTILISATION
(KO) 파에오닥틸룸 트리코르누툼의 신규 프로모터 HASP1와 이의 신호 펩타이드 및 이의 용도
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Abstract
(EN)
The present invention relates to a novel promoter HASP1 for inducing overexpression of a protein in Phaeodactylum tricornutum; a signal peptide HASP1-SP, which is a novel protein secretion sequence for inducing the secretion of the expressed protein; an expression vector comprising the HASP1 and the HASP1-SP; a transformant in which the expression vector has been introduced into a host cell; a method for producing a protein of interest comprising a step of culturing the transformant to obtain a culture; a method for expressing a protein of interest comprising a step of transforming the expression vector into a host cell; and a method for increasing the expression level of a protein of interest in a host cell comprising the step of transforming the expression vector into the host cell. The present invention is expected to be used as a powerful genetic tool capable of significantly increasing the productivity of a recombinant protein by using a novel promoter HASP1 for inducing overexpression of a protein in Phaeodactylum tricornutum , a signal peptide HASP1-SP which is a novel protein secretion sequence for inducing the secretion of the expressed protein, and an expression vector comprising the HASP1 and the HASP1-SP according to the present invention.
(FR)
La présente invention concerne un nouveau promoteur HASP1 pour Induire la surexpression d'une protéine dans Phaeodactylum tricornutum; un peptide signal HASP1-SP, qui est une nouvelle séquence de sécrétion de protéine pour induire la sécrétion de la protéine exprimée; un vecteur d'expression comprenant le HASP1 et le HASP1-SP; un transformant dans lequel le vecteur d'expression a été introduit dans une cellule hôte; un procédé de production d'une protéine d'intérêt comprenant une étape de culture du transformant pour obtenir une culture; un procédé d'expression d'une protéine d'intérêt comprenant une étape de transformation du vecteur d'expression en une cellule hôte; et un procédé pour augmenter le niveau d'expression d'une protéine d'intérêt dans une cellule hôte comprenant l'étape de transformation du vecteur d'expression dans la cellule hôte. La présente invention est censée être utilisée comme outil génétique puissant capable d'augmenter significativement la productivité d'une protéine recombinée utilisant un nouveau promoteur HASP1 pour induire une surexpression d'une protéine dans Phaeodactylum tricornutum , un peptide signal HASP1-SP qui est une nouvelle séquence de sécrétion de protéine pour induire la sécrétion de la protéine exprimée, et un vecteur d'expression comprenant Le HASP1 et le HASP1-SP selon la présente invention.
(KO)
본 발명은 파에오닥틸룸 트리코르누툼에서 단백질의 과발현을 유도하는 신규 프로모터 HASP1, 상기 발현된 단백질의 분비를 유도하는 신규 단백질 분비서열인 신호 펩타이드 HASP1-SP, 상기 HASP1 및 HASP1-SP를 포함하는 발현벡터, 발현벡터가 숙주세포에 도입된 형질전환체, 상기 형질전환체를 배양하여 배양물을 수득하는 단계를 포함하는 목적 단백질의 생산방법, 상기 발현벡터를 숙주세포에 형질전환하는 단계를 포함하는 목적 단백질을 발현시키는 방법 및 상기 발현벡터를 숙주세포에 형질전환하는 단계를 포함하는 숙주세포에서 목적 단백질의 발현량을 증가시키는 방법에 관한 것이다. 본 발명의 파에오닥틸룸 트리코르누툼(Phaeodactylum tricornutum)에서 단백질의 과발현을 유도하는 신규 프로모터 HASP1, 상기 발현된 단백질의 분비를 유도하는 신규 단백질 분비서열인 신호 펩타이드 HASP1-SP, 및 상기 HASP1 및 HASP1-SP를 포함하는 발현벡터를 이용하여 재조합 단백질의 생산성을 현저히 증대시킬 수 있는 강력한 유전자 도구로 사용될 수 있을 것으로 기대된다.
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