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1. WO2020237781 - METHOD FOR INCREASING CAPTURE RATE OF TRACE NUCLEIC ACID IN CELL-FREE BODY FLUID

Publication Number WO/2020/237781
Publication Date 03.12.2020
International Application No. PCT/CN2019/094963
International Filing Date 07.07.2019
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
C12N 15/1006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
Applicants
  • 浙江大学 ZHEJIANG UNIVERSITY [CN]/[CN]
Inventors
  • 黄彦钦 HUANG, Yanqin
  • 宋永茂 SONG, Yongmao
  • 袁瑛 YUAN, Ying
  • 郑树 ZHENG, Shu
Agents
  • 杭州求是专利事务所有限公司 HANGZHOU QIUSHI PATENT OFFICE CO., LTD.
Priority Data
201910451855.928.05.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD FOR INCREASING CAPTURE RATE OF TRACE NUCLEIC ACID IN CELL-FREE BODY FLUID
(FR) PROCÉDÉ POUR AUGMENTER LA VITESSE DE CAPTURE D'ACIDE NUCLÉIQUE À L'ÉTAT DE TRACE DANS UN FLUIDE CORPOREL ACELLULAIRE
(ZH) 一种提高无细胞体液中微量核酸捕获率的方法
Abstract
(EN)
Provided is a method for increasing the capture rate of trace nucleic acid in a cell-free body fluid. A step of centrifugation using a low dielectric constant solution is added between proteolysis and column chromatography binding.
(FR)
L'invention concerne un procédé pour augmenter le taux de capture d'acide nucléique à l'état de trace dans un fluide corporel acellulaire. Une étape de centrifugation à l'aide d'une solution à faible constante diélectrique est ajoutée entre la protéolyse et la liaison de chromatographie sur colonne.
(ZH)
提供了一种提高无细胞体液中微量核酸捕获率的方法,其中在蛋白裂解和过柱结合之间加入了使用低介电常数溶液离心的步骤。
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