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1. WO2020227206 - VECTORS AND EXPRESSION SYSTEMS FOR PRODUCING RECOMBINANT PROTEINS

Publication Number WO/2020/227206
Publication Date 12.11.2020
International Application No. PCT/US2020/031309
International Filing Date 04.05.2020
IPC
C12N 15/52 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
C12N 15/62 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
C12N 15/67 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 15/79 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
Applicants
  • AMGEN INC. [US]/[US]
Inventors
  • GHATTYVENKATAKRISHNA, Pavan
  • BARKHORDARIAN, Hedieh
  • DARIS, Kristine Marie
  • TEJAMO, Charilyn
Priority Data
62/844,36007.05.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) VECTORS AND EXPRESSION SYSTEMS FOR PRODUCING RECOMBINANT PROTEINS
(FR) VECTEURS ET SYSTÈMES D'EXPRESSION POUR PRODUIRE DES PROTÉINES RECOMBINÉES
Abstract
(EN)
Inventions disclosed herein relates to vectors and expression systems for producing heteromeric recombinant proteins such as monoclonal antibodies. Vectors and expression systems disclosed herein are based on the finding that the selectable marker glutamine synthetase can be divided into two fragments at selected amino acid positions of the glutamine synthetase polypeptide, and the two fragments can internet and/or associate to form a monomer and then a functional multimeric glutamine synthetase protein. Z
(FR)
L'invention concerne des vecteurs et des systèmes d'expression pour produire des protéines recombinées hétéromères telles que des anticorps monoclonaux. Les vecteurs et les systèmes d'expression décrits dans la présente invention sont basés sur la découverte selon laquelle la glutamine synthétase, en tant que marqueur sélectionnable, peut être divisée en deux fragments à des positions d'acides aminés sélectionnées du polypeptide glutamine synthétase, et les deux fragments peuvent s’intégrer et/ou s'associer pour former un monomère et ensuite une protéine de glutamine synthétase multimère fonctionnelle. Z
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