WO2020223104 ENGINEERED GLUCOSE DEHYDROGENASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS

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Publication Number WO/2020/223104

Publication Date 05.11.2020

International Application No. PCT/US2020/029517

International Filing Date 23.04.2020

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CPC

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Applicants

CODEXIS, INC. [US]/[US]

Inventors

YI, Xiang
ALVIZO, Oscar
GARCIA, Ravi, David
ENTWISTLE, David
CHING, Charlene
RIGGINS, James, Nicholas
SUBRAMANIAN, Nandhitha

Agents

NEELY WILLIS, Melanie

Priority Data

62/841,666

01.05.2019

Publication Language English (en)

Filing Language English (EN)

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Title

(EN) ENGINEERED GLUCOSE DEHYDROGENASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS

(FR) GLUCOSE DÉSHYDROGÉNASES MODIFIÉES ET PROCÉDÉS D'AMINATION RÉDUCTRICE DE COMPOSÉS CÉTONIQUES ET AMINÉS

Abstract

(EN) The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

(FR) La présente invention concerne des polypeptides de glucose déshydrogénase modifiés ayant une activité imine réductase, des polynucléotides codant pour les polypeptides modifiés, des cellules hôtes pouvant exprimer les polypeptides modifiés, et des procédés d'utilisation de ces polypeptides modifiés avec une gamme de composés de substrats cétoniques et aminés pour préparer des composés de produits aminés secondaires et tertiaires.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02	Oxidoreductases (1.), e.g. luciferase
04	acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
11	DNA or RNA fragments; Modified forms thereof
52	Genes encoding for enzymes or proenzymes
53	Oxidoreductases (1)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02	Oxidoreductases (1.), e.g. luciferase
04	acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

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1. WO2020223104 - ENGINEERED GLUCOSE DEHYDROGENASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Y	ENZYMES
101	Oxidoreductases acting on the CH-OH group of donors (1.1)
01	with NAD+ or NADP+ as acceptor (1.1.1)
01047	Glucose 1-dehydrogenase (1.1.1.47)