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1. WO2020222763 - MICROFLUIDIC REACTION CHAMBER FOR AMPLIFICATION OF NUCLEIC ACIDS

Publication Number WO/2020/222763
Publication Date 05.11.2020
International Application No. PCT/US2019/029707
International Filing Date 29.04.2019
IPC
C12M 1/34 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/36 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
36including condition or time responsive control, e.g. automatically controlled fermentors
C12M 3/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
C12Q 1/6844 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
B81B 1/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
81MICROSTRUCTURAL TECHNOLOGY
BMICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
1Devices without movable or flexible elements, e.g. microcapillary devices
Applicants
  • HEWLETT-PACKARD DEVELOPMENT COMPANY L.P. [US]/[US]
Inventors
  • CHOY, Si-lam
  • ELY, Hilary
  • LAHMANN, John
Agents
  • COSTALES, Shruti
  • FENSKE, Elizabeth A.
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MICROFLUIDIC REACTION CHAMBER FOR AMPLIFICATION OF NUCLEIC ACIDS
(FR) CHAMBRE DE RÉACTION MICROFLUIDIQUE POUR AMPLIFICATION D'ACIDES NUCLÉIQUES
Abstract
(EN)
Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit
(FR)
Des exemples de l'invention impliquent l'amplification et la détection d'acides nucléiques à l'aide d'une chambre de réaction microfluidique. Un appareil donné à titre d'exemple comprend un circuit de chambre de réaction pour traiter un réactif et un échantillon biologique pour l'amplification d'acides nucléiques. L'appareil comprend en outre une pluralité de capillaires pour faire passer le réactif et l'échantillon biologique à travers la chambre de réaction microfluidique. Un système de commande de vanne peut commander sélectivement chaque vanne d'une pluralité de vannes pour amener le réactif et l'échantillon biologique à se déplacer sélectivement à travers la chambre de réaction microfluidique pour l'amplification des acides nucléiques selon une séquence de synchronisation particulière. Dans divers exemples, une région de piégeage disposée dans la chambre de réaction microfluidique fixe les acides nucléiques dans la chambre de réaction microfluidique pour une amplification à l'aide du circuit de chambre de réaction
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