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1. WO2020221579 - PROTEASES WITH IMPROVED ENZYME STABILITY IN DETERGENTS AND CLEANING AGENTS III

Publication Number WO/2020/221579
Publication Date 05.11.2020
International Application No. PCT/EP2020/060385
International Filing Date 14.04.2020
IPC
C12N 9/54 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50Proteinases
52derived from bacteria
54bacteria being Bacillus
C11D 11/00 2006.01
CCHEMISTRY; METALLURGY
11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
11Special methods for preparing compositions containing mixtures of detergents
CPC
C11D 11/0017
CCHEMISTRY; METALLURGY
11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
DDETERGENT COMPOSITIONS
11Special methods for preparing compositions containing mixtures of detergents
0005Special cleaning and washing methods
0011characterised by the objects to be cleaned
0017"Soft" surfaces, e.g. textiles
C12N 9/54
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
48acting on peptide bonds (3.4)
50Proteinases ; , e.g. Endopeptidases (3.4.21-3.4.25)
52derived from bacteria ; or Archaea
54bacteria being Bacillus
C12Y 304/21062
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
304Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
21Serine endopeptidases (3.4.21)
21062Subtilisin (3.4.21.62)
Applicants
  • HENKEL AG & CO. KGAA [DE]/[DE]
Inventors
  • MUSSMANN, Nina
  • WIELAND, Susanne
  • DEGERING, Christian
  • FERNANDEZ, Layla
  • GRIEMERT, Sabine
  • PEIFFER, Barbara
  • PULS, Michael
  • PRUESER, Inken
Agents
  • VIERING, JENTSCHURA & PARTNER MBB
Priority Data
10 2019 111 057.229.04.2019DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) PROTEASEN MIT VERBESSERTER ENZYMSTABILITÄT IN WASCH- UND REINIGUNGSMITTELN III
(EN) PROTEASES WITH IMPROVED ENZYME STABILITY IN DETERGENTS AND CLEANING AGENTS III
(FR) PROTÉASES PRÉSENTANT UNE STABILITÉ ENZYMATIQUE AMÉLIORÉE DANS DES DÉTERGENTS ET PRODUITS DE NETTOYAGE III
Abstract
(DE)
Die Erfindung betrifft Proteasen umfassend eine Aminosäuresequenz, die mindestens 70 % Sequenzidentität mit der in SEQ ID NO: 1 angegebenen Aminosäuresequenz über deren Gesamtlänge aufweist und jeweils bezogen auf die Nummerierung gemäß SEQ ID NO: 1 (i) an den Positionen, die den Positionen 9, 144, 252 und 271 entsprechen, Aminosäuresubstitutionen aufweist, vorzugsweise ausgewählt aus den Aminosäuresubstitutionen 9T, 144K, 252T und 271E; sowie (ii) an mindestens einer der Positionen, die den Positionen 53, 120, 131, 149, 159, 162, 166, 172, 189, 192, 211, 215, 217, 224 und 274 entsprechen, mindestens eine weitere Aminosäuresubstitution aufweist; sowie deren Herstellung und Verwendung. Derartige Proteasen zeigen eine sehr gute Stabilität bei guter Reinigungsleistung.
(EN)
The invention relates to proteases comprising an amino acid sequence which shares at least 70 % sequence identity with the amino acid sequence given in SEQ ID NO: 1, over their entire length, and, with respect to the numbering according to SEQ ID NO: 1 (i) has amino acid substitutions at positions corresponding to positions 9, 144, 252 and 271, preferably selected from among the amino acid substitutions 9T, 144K, 252T and 271E; and (ii) has at least one additional amino acid substitution at least at one of the positions corresponding to positions 53, 120, 131, 149, 159, 162, 166, 172, 189, 192, 211, 215, 217, 224 and 274. The invention also relates to the production and use of said proteases. Proteases of this type exhibit very good stability with a good cleaning performance.
(FR)
L'invention concerne des protéases comprenant une séquence d'acides aminés présentant sur la longueur totale une identité de séquence au moins égale à 70% à la séquence d'acides aminés indiquée dans SEQ ID NO: 1 et présentant des substitutions d'acides aminés (i) au niveau des positions correspondant aux positions 9, 144, 252 et 271 respectivement par rapport à la numérotation selon SEQ ID NO: 1, de préférence sélectionnées parmi les substitutions d'acides aminés 9T, 144K, 252T et 271E; et (ii) présentant au moins une substitution d'acides aminés supplémentaire, au moins au niveau d'une des positions correspondant aux positions 53, 120, 131, 149, 159, 162, 166, 172, 189, 192, 211, 215, 217, 224 et 274. Cette invention concerne en outre la production et l'utilisation de ces protéases. Les protéases selon l'invention présentent une très bonne stabilité et de bonnes performances de nettoyage.
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