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1. WO2020221314 - TUMOR MARKER STAMP-EP7 BASED ON METHYLATED MODIFICATION AND USE THEREOF

Publication Number WO/2020/221314
Publication Date 05.11.2020
International Application No. PCT/CN2020/087883
International Filing Date 29.04.2020
IPC
C12N 15/11 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12Q 1/6886 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
6886for cancer
CPC
C12Q 1/6886
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
6886for cancer
C12Q 2600/154
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
154Methylation markers
Applicants
  • 上海奕谱生物科技有限公司 SHANGHAI EPIPROBE BIOTECHNOLOGY CO., LTD. [CN]/[CN]
Inventors
  • 李振艳 LI, Zhenyan
  • 罗怀兵 LUO, Huaibing
Agents
  • 上海专利商标事务所有限公司 SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC
Priority Data
201910363081.430.04.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) TUMOR MARKER STAMP-EP7 BASED ON METHYLATED MODIFICATION AND USE THEREOF
(FR) MARQUEUR TUMORAL STAMP-EP7 BASÉ SUR UNE MODIFICATION DE MÉTHYLATION ET SON UTILISATION
(ZH) 基于甲基化修饰的肿瘤标记物STAMP-EP7及其应用
Abstract
(EN)
Provided are an isolated polynucleotide and a use thereof, wherein the polynucleotide is a polynucleotide as shown in SEQ ID NO:1, a fragment with a modified CpG site, or a complementary nucleic acid. Also provided are the use of the polynucleotide for preparing a tumor detection reagent or a kit, a preparation method therefor, a reagent and a use thereof, a kit and a method for detecting the methylation profile of samples in vitro.
(FR)
L'invention concerne un polynucléotide isolé et son utilisation, le polynucléotide étant un polynucléotide tel que présenté dans SEQ ID NO : 1, un fragment ayant un site CpG modifié, ou un acide nucléique complémentaire. L'invention concerne également l'utilisation du polynucléotide pour préparer un réactif de détection de tumeur ou un kit, son procédé de préparation, un réactif et une utilisation de celui-ci, un kit et un procédé pour détecter le profil de méthylation d'échantillons in vitro.
(ZH)
提供了一种分离的多核苷酸及其用途,其是SEQ ID NO:1所示多核苷酸或其存在修饰的CpG位点的片段或互补核酸。还提供了多核苷酸用于制备肿瘤检测试剂或试剂盒的用途,制备方法,试剂及其用途,试剂盒,以及体外检测样品的甲基化谱式的方法。
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