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1. WO2020221153 - ZIPPER FASTENER STRUCTURE OF PROMOTING FORMATION OF PROTEIN DIMER AND APPLICATION THEREOF

Publication Number WO/2020/221153
Publication Date 05.11.2020
International Application No. PCT/CN2020/086975
International Filing Date 26.04.2020
IPC
C07K 19/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
19Hybrid peptides
C07K 14/35 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
35from Mycobacteriaceae (F)
C12N 15/62 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
C12N 15/63 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/564 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
564for pre-existing immune complex or autoimmune disease
Applicants
  • 广州市雷德生物科技有限公司 LEIDE BIOSCIENCES CO., LTD. [CN]/[CN]
Inventors
  • 杨翔 YANG, Xiang
  • 楼建荣 LOU, Jianrong
  • 谢桂华 XIE, Guihua
Agents
  • 广州嘉权专利商标事务所有限公司 JIAQUAN IP LAW FIRM
Priority Data
201910348168.428.04.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) ZIPPER FASTENER STRUCTURE OF PROMOTING FORMATION OF PROTEIN DIMER AND APPLICATION THEREOF
(FR) STRUCTURE DE FERMETURE À GLISSIÈRE PERMETTANT DE FAVORISER LA FORMATION D'UN DIMÈRE PROTÉIQUE ET SON UTILISATION
(ZH) 一种促进蛋白二聚体形成的拉链扣结构及其应用
Abstract
(EN)
The present invention relates to the field of genetic engineering, and provides a zipper fastener structure of promoting formation of a protein dimer and application thereof. The zipper fastener can be applied to dimerization of proteins of the same type and dimerization of proteins of different types, and can also be applied to polypeptide cycle formation, polypeptide dimerization, and polypeptide extension. A ESAT6-CFP10 dimer having an approximately native conformation can be obtained, and the dimer has better solubility, and has a better stimulating effect on memory T cells than a ESAT6-CFP10 protein capable of linear fusion expression. A dimer zipper fastener can assist the formation of a more stable cyclic polypeptide, and a CCP polypeptide added with a dimer fastener can improve the detection rate for citrullinated autoantibodies in serum of a rheumatoid arthritis patient.
(FR)
La présente invention se rapporte au domaine du génie génétique, et concerne une structure de fermeture à glissière permettant de favoriser la formation d'un dimère protéique et son utilisation. La fermeture à glissière peut être appliquée à la dimérisation de protéines du même type et à la dimérisation de protéines de différents types, et peut également être appliquée à la formation de cycle polypeptidique, à la dimérisation de polypeptide et à l'extension de polypeptide. L'invention permet d'obtenir un dimère ESAT6-CFP10 ayant une conformation approximativement native, le dimère ayant une meilleure solubilité, et un meilleur effet de stimulation sur des lymphocytes T mémoire par comparaison avec une protéine ESAT6-CFP10 capable d'une expression de fusion linéaire. Une fermeture à glissière à base de dimère peut aider à la formation d'un polypeptide cyclique plus stable, et un polypeptide CCP ajouté à une fermeture dimère peut améliorer le taux de détection d'auto-anticorps citrullinés dans le sérum d'un patient atteint de polyarthrite rhumatoïde.
(ZH)
本发明属于基因工程领域,提供一种促进蛋白二聚体形成的拉链扣结构及其应用,该拉链扣可用于同种蛋白的二聚,异种蛋白的二聚,也可用于多肽成环,二聚多肽及多肽的延伸。可以得到接近天然构象的ESAT6-CFP10二聚体,该二聚体具有更好的可溶性,对记忆性T细胞刺激效果要好于线性融合表达的ESAT6-CFP10蛋白。二聚体拉链扣可帮助形成更稳定的环型多肽,添加了二聚体扣的CCP多肽可以增加对类风湿关节炎病人血清中瓜氨酸化自身抗体的检出率。
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