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1. WO2020206114 - SEQUENCING BY SYNTHESIS WITH ENERGY TRANSFER DYE PAIRS

Publication Number WO/2020/206114
Publication Date 08.10.2020
International Application No. PCT/US2020/026378
International Filing Date 02.04.2020
IPC
C12N 9/12 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
C12Q 1/6818 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6818involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6869 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
Applicants
  • THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK [US]/[US] (AllExceptUS)
  • JU, Jingyue [US]/[US] (US)
  • KUMAR, Shiv [US]/[US] (US)
  • RUSSO, James [US]/[US] (US)
  • LI, Xiaoxu [CN]/[US] (US)
  • JOCKUSCH, Steffen [DE]/[US] (US)
  • CHIEN, Minchen [US]/[US] (US)
  • TAO, Chuanjuan [US]/[US] (US)
Inventors
  • JU, Jingyue
  • KUMAR, Shiv
  • RUSSO, James
  • LI, Xiaoxu
  • JOCKUSCH, Steffen
  • CHIEN, Minchen
  • TAO, Chuanjuan
Agents
  • GERSHIK, Gary J.
Priority Data
62/828,03102.04.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SEQUENCING BY SYNTHESIS WITH ENERGY TRANSFER DYE PAIRS
(FR) SÉQUENÇAGE PAR SYNTHÈSE AVEC DES PAIRES DE COLORANTS DE TRANSFERT D'ÉNERGIE
Abstract
(EN)
The invention provides nucleotide analogues and methods of use thereof for sequencing by synthesis using energy transfer dye pairs. In an embodiment of the invention, dye on the fluorescently labeled nucleotide analogues is an energy transfer donor dye and the dye on the second nucleotide polymerase is an energy transfer acceptor dye, or wherein the dye on the fluorescently labeled nucleotide analogues is an energy transfer acceptor dye and the dye on the second nucleotide polymerase is an energy transfer donor dye.
(FR)
L'invention concerne des analogues de nucléotides et des procédés d'utilisation de ces derniers pour le séquençage par synthèse à l'aide de paires de colorants de transfert d'énergie. Dans un mode de réalisation de l'invention, le colorant sur les analogues nucléotidiques marqués par fluorescence est un colorant donneur de transfert d'énergie et le colorant sur la seconde polymérase nucléotidique est un colorant accepteur de transfert d'énergie, ou le colorant sur les analogues nucléotidiques marqués par fluorescence est un colorant accepteur de transfert d'énergie et le colorant sur la seconde polymérase nucléotidique est un colorant donneur de transfert d'énergie.
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