Processing

Please wait...

Settings

Settings

Goto Application

1. WO2020202070 - METHOD TO DETECT AND ENUMERATE MICROORGANISMS

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

[ EN ]

What is claimed is:

1. A method, comprising

providing a device, the device comprising:

a body member comprising a self-supporting, waterproof substrate having upper and lower surfaces; and

a layer of adhesive-coated on the upper surface of the substrate, the adhesive being non-inhibitory to the growth of microorganisms; and a cold-water-soluble powder adhered to the adhesive, the powder comprising:

guar gum;

one or more nutrients for growing microorganisms; and

a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member;

incubating a sample in the device to form a gel; and

counting colonies between 24 hours to 72 hours and/or determining if the gel surrounding a colony is degraded.

3. The method of claim 1 or 2, wherein the cover sheet comprises a transparent film.

4. The method of claim 3, wherein the film is selected from the group consisting of polyester, polyethylene, polypropylene, polystyrene and silicone.

5. The method of any of claims 1 to 4, wherein the device further comprises a hydrophobic spacer element adhered to the upper surface of the substrate forming side walls to retain a predetermined amount of liquid in contact with the substrate.

6. The method of claim 5, wherein the spacer element comprises a hydrophobic foam sheet having a hole therein.

7. The method of claim 6, wherein the foam is polystyrene.

8. The method of any of claims 1 to 7, wherein the substrate is a film selected from the group consisting of polyester, polypropylene, polyethylene and polystyrene.

9. The method of any of claims 1 to 8, wherein the substrate has a grid pattern printed thereon.

10. The method of any of claims 1 to 9, wherein the adhesive is a pressure-sensitive adhesive.

11. The method of any of claims 1 to 10, wherein the adhesive is substantially transparent when wetted with water.

12. The method of any of claims 1 to 11, wherein the adhesive is a copolymer of isooctyl acrylate and acrylamide in a mole ratio of 94:6, respectively.

13. The method of any of claims 1 to 12, wherein one of the powder and the adhesive contains a dye which is metabolizable by microorganisms and which causes the microorganisms and/or the surrounding gel to be colored or fluorescent.

14. The method of any of claims 1 to 13, wherein the dye is selected from the group consisting of triphenyltetrazolium chloride, p-tolyltetrazolium red, tetrazolium violet and veratryltetrazoleum blue.