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1. WO2020202070 - METHOD TO DETECT AND ENUMERATE MICROORGANISMS

Publication Number WO/2020/202070
Publication Date 08.10.2020
International Application No. PCT/IB2020/053156
International Filing Date 02.04.2020
IPC
C09K 8/66 2006.01
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
KMATERIALS FOR APPLICATIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
8Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
60Compositions for stimulating production by acting on the underground formation
62Compositions for forming crevices or fractures
66Compositions based on water or polar solvents
C12M 1/16 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
16containing, or adapted to contain, solid media
C12Q 1/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
CPC
C09K 8/66
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
8Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
60Compositions for stimulating production by acting on the underground formation
62Compositions for forming crevices or fractures
66Compositions based on water or polar solvents
C12M 33/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
33Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
02by impregnation, e.g. using swabs or loops
C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Applicants
  • 3M INNOVATIVE PROPERTIES COMPANY [US]/[US]
Inventors
  • BRUTINEL, Evan D.
Agents
  • HAN, Qiang
  • FLORCZAK, Yen Tong,
  • BLANK, Colene H.,
  • GEISE, C. Michael,
  • HARTS, Dean M. ,
  • LEVINSON, Eric D.,
  • NOWAK, Sandra K.,
  • RINGSRED, Ted K.,
Priority Data
62/830,05805.04.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD TO DETECT AND ENUMERATE MICROORGANISMS
(FR) PROCÉDÉ DE DÉTECTION ET DE DÉNOMBREMENT DE MICROORGANISMES
Abstract
(EN)
A method. The method includes providing a device, the device comprising: a body member comprising a self-supporting, waterproof substrate having upper and lower surfaces; and a layer of adhesive-coated on the upper surface of the substrate, the adhesive being non-inhibitory to the growth of microorganisms; and a cold-water-soluble powder adhered to the adhesive, the powder comprising: guargum; one or more nutrients for growing microorganisms; and a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; incubating a sample in the device to form a gel; and counting colonies between 24 hours to 72 hours and/or determining if the gel surrounding a colony is degraded.
(FR)
La présente invention se rapporte à un procédé. Le procédé comprend l'utilisation d'un dispositif, le dispositif comprenant : un élément de corps comprenant un substrat autonome étanche à l'eau comportant des surfaces supérieure et inférieure ; et une couche d'adhésif recouvrant la surface supérieure du substrat, l'adhésif n'inhibant pas la croissance des microorganismes ; et une poudre soluble dans l'eau froide collée à l'adhésif, la poudre comprenant : de la gomme de guar ; un ou plusieurs nutriments pour la culture des microorganismes ; et une feuille protectrice comportant une surface tournée vers l'intérieur et une surface tournée vers l'extérieur, la feuille protectrice adhérant à au moins une partie de l'élément de corps ; l'incubation d'un échantillon dans le dispositif pour former un gel ; et le comptage des colonies entre 24 heures et 72 heures et/ou la détermination de la dégradation ou pas du gel entourant une colonie.
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