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1. WO2020201350 - MEANS AND METHODS FOR SINGLE MOLECULE PEPTIDE SEQUENCING

Publication Number WO/2020/201350
Publication Date 08.10.2020
International Application No. PCT/EP2020/059250
International Filing Date 01.04.2020
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 33/6818
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6818Sequencing of polypeptides
G01N 33/6824
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6818Sequencing of polypeptides
6824involving N-terminal degradation, e.g. Edman degradation
Applicants
  • VIB VZW [BE]/[BE]
  • UNIVERSITEIT GENT [BE]/[BE]
Inventors
  • CALLEWAERT, Nico
  • DEVOS, Simon
  • EYCKERMAN, Sven
  • VANDEKERCKHOVE, Joel
Common Representative
  • VIB VZW
Priority Data
1904697.803.04.2019GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MEANS AND METHODS FOR SINGLE MOLECULE PEPTIDE SEQUENCING
(FR) MOYENS ET MÉTHODES DE SÉQUENÇAGE DES PEPTIDES D'UNE SEULE MOLÉCULE
Abstract
(EN)
The present application relates to the field of protein sequencing, more particularly to protein profiling using massively parallel sequencing with single-molecule sensitivity. Methods, assays and reagents are provided for sequencing individual protein or polypeptide molecules. Also provided are methods and assays for the parallel sequencing of proteins or polypeptides. To this end, particular labeled probes are used that are reactive with the N-terminal amino acid of the polypeptide molecules and can be detected while still associated with the polypeptide(s).
(FR)
La présente invention se rapporte au domaine du séquençage de protéines, plus particulièrement au profilage de protéines à l'aide d'un séquençage massivement parallèle avec une sensibilité à une molécule unique. L'invention concerne des méthodes, des dosages et des réactifs permettant de séquencer des molécules individuelles de protéines ou de polypeptides. L'invention concerne également des méthodes et des dosages de séquençage parallèle de protéines ou de polypeptides. À cet effet, l'invention utilise des sondes marquées particulières réactives avec l'acide aminé N-terminal des molécules polypeptidiques et pouvant être détectées tout en étant encore associées au(x) polypeptide(s).
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