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1. WO2020201147 - MICROPLATE FOR MICROSCOPY

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[ EN ]

CLAIMS

1. A microplate (10) for microscopy of an organoid (30), comprising: a body (1 1 ) having at least one recess (20), the recess (20) being adapted to contain and restrict movement of the organoid (30), and

a reflective surface (40),

characterized in that the reflective surface (40) is inclined in relation to the recess (20), such that an incoming beam of light towards the microplate (10) is directed onto the organoid (30) in a substantially horizontal direction.

2. A microplate (10) according to claim 1 , wherein the reflective surface

(40) is formed between a first volume (41 ) having a first refractive index and a second volume (42) having a second refractive index.

3. A microplate (10) according to claim 2, wherein the first refractive index is larger than the second refractive index.

4. A microplate (10) according to claim 2 or 3, wherein the first volume

(41 ) is a portion of the body (1 1 ) having a first refractive index and the second volume (42) is a portion of the body (1 1 ) having a second refractive index.

5. A microplate (10) according to claim 2 to 4, wherein the first volume (41 ) is made of a first material and the second volume (42) is made of a second material.

6. A microplate (10) according to claim 2, wherein the first volume (41 ) is a portion of the body (1 1 ) and the second volume (42) is constituted by surrounding medium present in a groove (42) in the bottom of the body (1 1 ).

7. A microplate (10) according to claim 6, wherein the groove (42) is formed in a triangular shape.

8. A microplate (10) according to any of the preceding claims, wherein the recess (20) is formed as cylinder or as a prism.

9. A microplate (10) according to any of the preceding claims, wherein

the reflective surface (40) is arranged such that each point of the organoid (30) may be illuminated by a beam of light incoming towards a corresponding point of the reflective surface (40).

10. A microplate (10) according to any of the preceding claims, wherein the reflective surface (40) is inclined relative the recess (20) by 45 degrees.

1 1. A microplate (10) according to any of the preceding claims, wherein the recess (20) comprises a first portion (21 ) being adapted to contain and restrict movement of the organoid (30) by having a horizontal cross sectional area such that the movement of the organoid (30) is restricted.

12. A microplate (10) according to claim 11 , wherein the recess (20) comprises:

a second portion (22) being adapted to receive a pipette,

wherein the second portion (22) has a horizontal cross sectional area such that it may receive a pipette.

13. A microplate (10) according to any of the preceding claims, wherein the body (1 1 ) is made of a transparent material.

14. Method for microscopy of an organoid (30) in a microplate (10), the microplate (10) comprising:

a body (1 1 ) having at least one recess (20), the recess (20) being adapted to contain and restrict movement of the organoid (30), and

a reflective surface (40), the reflective surface (40) being inclined in relation to the recess (20), such that an incoming beam of light towards the microplate (10) is directed onto the organoid (30) in a substantially horizontal direction

characterized by the steps of:

placing the organoid (30) in the recess (20),

illuminating a portion of the organoid (30) from a substantially horizontal direction by sending a beam of light onto the reflective surface (40) of the microplate (10),

capturing the light signal from the illuminated portion of the organoid (30) to form an image of the illuminated portion of the organoid (30).

15. Method according to claim 14, further comprising the steps of:

stepwise illuminating a plurality of portions of the organoid (30) from a substantially horizontal direction by stepwise sending a beam of light onto a corresponding portion of the reflective surface (40) of the microplate (10), and stepwise capturing the signal from the plurality of illuminated portions of the organoid (30) to form an image of the plurality of illuminated portions of the organoid (30).